Speeds of actin translocation in vitro by myosins extracted from single rat muscle fibres of different types

被引：25

作者：

Canepari, M ^{[1
]}

Rossi, R ^{[1
]}

Pellegrino, MA ^{[1
]}

Reggiani, C ^{[1
]}

Bottinelli, R ^{[1
]}

机构：

[1] Univ Pavia, Inst Human Physiol, I-27100 Pavia, Italy

来源：

EXPERIMENTAL PHYSIOLOGY | 1999年 / 84卷 / 04期

关键词：

D O I：

10.1111/j.1469-445X.1999.00350.x

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

As skeletal muscle fibres mostly express a single myosin isoform, they are a potential source of pure myosin isoforms. A technique is described that allows extraction and identification of pure myosin isoforms from single fibres, and testing of such myosins in an in vitro motility assay (IVMA). The results show that the extraction procedure does not alter myosin function and support the view that single fibres are reliable sources of purified myosin isoforms for IVMA.

引用

收藏

页码：803 / 806

页数：4

相关论文

共 12 条

[1] ACTOMYOSIN KINETICS AND IN-VITRO MOTILITY OF WILD-TYPE DROSOPHILA ACTIN AND THE EFFECTS OF 2 MUTATIONS IN THE ACT88F GENE [J].

ANSON, M ;

DRUMMOND, DR ;

GEEVES, MA ;

HENNESSEY, ES ;

RITCHIE, MD ;

SPARROW, JC .

BIOPHYSICAL JOURNAL, 1995, 68 (05) :1991-2003

[2] UNLOADED SHORTENING VELOCITY AND MYOSIN HEAVY-CHAIN AND ALKALI LIGHT-CHAIN ISOFORM COMPOSITION IN RAT SKELETAL-MUSCLE FIBERS [J].

BOTTINELLI, R ;

BETTO, R ;

SCHIAFFINO, S ;

REGGIANI, C .

JOURNAL OF PHYSIOLOGY-LONDON, 1994, 478 (02) :341-349

[3] The in vitro motility activity of beta-cardiac myosin depends on the nature of the beta-myosin heavy chain gene mutation in hypertrophic cardiomyopathy [J].

Cuda, G ;

Fananapazir, L ;

Epstein, ND ;

Sellers, JR .

JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, 1997, 18 (03) :275-283

[4] In vitro actin filament sliding velocities produced by mixtures of different types of myosin [J].

Cuda, G ;

Pate, E ;

Cooke, R ;

Sellers, JR .

BIOPHYSICAL JOURNAL, 1997, 72 (04) :1767-1779

[5] SMOOTH CARDIAC AND SKELETAL-MUSCLE MYOSIN FORCE AND MOTION GENERATION ASSESSED BY CROSS-BRIDGE MECHANICAL INTERACTIONS IN-VITRO [J].

HARRIS, DE ;

WORK, SS ;

WRIGHT, RK ;

ALPERT, NR ;

WARSHAW, DM .

JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY, 1994, 15 (01) :11-19

[6]

MARGOSSIAN SS, 1982, METHOD ENZYMOL, V85, P55

[7] THE FORCE-VELOCITY RELATION OF RAT FAST-TWITCH AND SLOW-TWITCH MUSCLES EXAMINED AT DIFFERENT TEMPERATURES [J].

RANATUNGA, KW .

JOURNAL OF PHYSIOLOGY-LONDON, 1984, 351 (JUN) :517-529

[8] Molecular diversity of myofibrillar proteins: Gene regulation and functional significance [J].

Schiaffino, S ;

Reggiani, C .

PHYSIOLOGICAL REVIEWS, 1996, 76 (02) :371-423

[9] Comparison of unitary displacements and forces between 2 cardiac myosin isoforms by the optical trap technique - Molecular basis for cardiac adaptation [J].

Sugiura, S ;

Kobayakawa, N ;

Fujita, H ;

Yamashita, H ;

Momomura, S ;

Chaen, ST ;

Omata, M ;

Sugi, H .

CIRCULATION RESEARCH, 1998, 82 (10) :1029-1034

[10] Kinetic tuning of myosin via a flexible loop adjacent to the nucleotide binding pocket [J].

Sweeney, HL ;

Rosenfeld, SS ;

Brown, F ;

Faust, L ;

Smith, J ;

Xing, J ;

Stein, LA ;

Sellers, JR .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (11) :6262-6270