Astrocytic Ca2+ signaling evoked by sensory stimulation in vivo

被引:501
作者
Wang, Xiaohai
Lou, Nanhong
Xu, Qiwu
Tian, Guo-Feng
Peng, Wei Guo
Han, Xiaoning
Kang, Jian
Takano, Takahiro
Nedergaard, Maiken
机构
[1] Univ Rochester, Med Ctr, Dept Neurosurg, Ctr Aging & Dev Biol, Rochester, NY 14642 USA
[2] New York Med Coll, Dept Cell Biol & Anat, Valhalla, NY 10595 USA
关键词
D O I
10.1038/nn1703
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Although astrocytes are the most abundant cell type in the brain, evidence for their activation during physiological sensory activity is lacking. Here we show that whisker stimulation evokes increases in astrocytic cytosolic calcium (Ca2+) within the barrel cortex of adult mice. Increases in astrocytic Ca2+ were a function of the frequency of stimulation, occurred within several seconds and were inhibited by metabotropic glutamate receptor antagonists. To distinguish between synaptic input and output, local synaptic activity in cortical layer 2 was silenced by iontophoresis of AMPA and NMDA receptor antagonists. The antagonists did not reduce astrocytic Ca2+ responses despite a marked reduction in excitatory postsynaptic currents in response to whisker stimulation. These findings indicate that astrocytes respond to synaptic input, by means of spillover or ectopic release of glutamate, and that increases in astrocytic Ca2+ occur independently of postsynaptic excitatory activity.
引用
收藏
页码:816 / 823
页数:8
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