Nitric oxide inhibits the HIV-1 reverse transcriptase activity

被引：41

作者：

Persichini, T

Colasanti, M

Fraziano, M

Colizzi, V

Medana, C

Polticelli, F

Venturini, G

Ascenzi, P

机构：

[1] Unie Rome Tre, Dept Biol, I-00146 Rome, Italy

[2] Univ Roma Tor Vergata, Dept Biol, I-00133 Rome, Italy

[3] Univ Turin, Dept Pharmaceut Chem & Technol, I-10125 Turin, Italy

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1999年 / 258卷 / 03期

关键词：

HIV-1 reverse transcriptase; nitric oxide; NO-donor; enzyme inhibition;

D O I：

10.1006/bbrc.1999.0581

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nitric oxide (NO) is a polypotent regulatory molecule involved in a variety of activities, such as the modulation of the catalytic activity of cysteine-containing enzymes. The present study reports the modulation of the HIV-1 reverse transcriptase activity by NO, released by the NO donors 3,3-bis(aminoethyl)-1-hydroxy-2-oxo-1-triazene (NOC-18), (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]5-nitro-3-hexenamide (NOR-3), 3-morpholinosydnonimine (SIN-1), 4-(phenylsulfonyl)-3-((2-(dimethylamino) ethyl)thio)furoxan oxalate (SNO-102), and sodium nitroprusside (SNP). NO inhibits dose-dependently the HIV-1 reverse transcriptase activity, Likely due to oxidation of Cys residue(s). Present results, representing a new insight into the modulation mechanism of the HIV-1 reverse transcriptase activity, may be relevant to develop new strategies for inhibition of HIV-1 replication. (C) 1999 Academic Press.

引用

页码：624 / 627

页数：4

共 28 条

[1] Enzyme inactivation through sulfhydryl oxidation by physiologic NO-carriers [J].