Preliminary investigation of gene expression profiles in peripheral blood lymphocytes in schizophrenia

被引:90
作者
Bowden, NA
Weidenhofer, J
Scott, RJ
Schall, U
Todd, J
Michie, PT
Tooney, PA [1 ]
机构
[1] Univ Newcastle, Sch Biomed Sci, Dept Expt Pharmacol, Callaghan, NSW 2308, Australia
[2] John Hunter Hosp, Hunter Neurosci & Hunter Med Res Inst, New Lambton Heights, NSW 2305, Australia
[3] John Hunter Hosp, Div Genet, Hunter Area Pathol Serv, New Lambton Heights, NSW 2305, Australia
[4] James Fletcher Hosp, Ctr Mental Hlth Studies, Newcastle, NSW 2300, Australia
[5] Univ Newcastle, Sch Behav Sci, Callaghan, NSW 2308, Australia
关键词
schizophrenia; classification; peripheral blood lymphocytes; gene expression; microarrays; age;
D O I
10.1016/j.schres.2005.11.012
中图分类号
R749 [精神病学];
学科分类号
100205 ;
摘要
Schizophrenia is a heterogenous disorder that is phenomenologically characterised by a combination of negative, positive, and cognitive symptoms with variable expression in the Course of illness. Here, we investigated differential gene expression in relation to age to address the heterogeneity of this disorder We used 6000 gene cDNA microarrays to generate gene expression profiles from peripheral blood lymphocytes from 14 individuals with schizophrenia and 14 non-psychiatric controls. Genes showing altered expression were identified and 18 genes with brain-related functions were altered, 4 of which, endothelial differentiation gene 2 (Edg-2), ezrin-radixin-moesin phosphoprotein 50 (EBP50), Myc-associated zinc finger protein (MAZ) and Tumor Necrosis Factor Receptor 2 (TNFR2), were confirmed by relative real-time PCR. Dendrograms were constructed using genes that showed significantly different expression (p < 0.05) between groups based on median split of age dividing the matched pairs into distinct Subclasses. Our findings Suggest that distinct gene expression profiles in peripheral blood lymphocytes associated with schizophrenia phenotypes may provide a first step towards the biological classification of schizophrenia Subtypes. The validity of this approach may lead to better methods of defining this enigmatic disease. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:175 / 183
页数:9
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