Identification of a Genetic Locus Related to Antivirus Production in Pseudomonas fluorescence strain Gpf01 Against Cucumber mosaic virus

被引：3

作者：

Cho, Saeyoull ^{[1
]}

Lee, Seon-Hwa ^{[1
]}

Park, Sujin ^{[2
]}

Choi, Kyu-Up ^{[1
]}

Cho, Junmo ^{[1
]}

Hur, Jang Hyun ^{[3
]}

Shrestha, Anupama ^{[1
]}

Lim, Chunkeun ^{[1
]}

机构：

[1] Kangwon Natl Univ, Coll Agr & Life Sci, Div Appl Biol, Dept Bioresource Technol, Chunchon 200701, South Korea

[2] Univ Michigan, Inst Life Sci, Ann Arbor, MI 48109 USA

[3] Kangwon Natl Univ, Coll Agr & Life Sci, Div Environm Biol, Chunchon 200701, South Korea

来源：

PLANT PATHOLOGY JOURNAL | 2009年 / 25卷 / 01期

关键词：

antiviral compound producing gene; Cucumber mosaic virus; Pseudomonas fluorescens; SYSTEMIC RESISTANCE; INDUCTION; COMPOUND; TOBACCO; TOMATO;

D O I：

10.5423/PPJ.2009.25.1.077

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Pseudomonas fluorescens strain Gpf01, isolated from ginseng rhizosphere showed antiviral activity against Cucumber mosaic virus, when tested in a local host of CMV, Chenopodium amaranticolor. Transposon mutant library of Gpf01 was prepared using pGS9::Tn5 and the mutant Gpf01-RS19 was found to loose antiviral production. We developed primers from the flanking region of Tn5 and found a cosmid clone pAV1123, harboring 1.2 kb antiviral compound producing (avcf01) locus. When a sub-clone pPH9, which carried 9.3 kb region of pAV1123, was introduced into antivirus deficient P. fluorescens wild type strain B16, it exhibited antiviral activity. Using Tn3-gus mutagenesis and complementation analysis, it was found that the genes related to antiviral activity production resided in a 9.3-kb HindIII-HindIII fragment of pAV1123, indicating that the plasmid carries an essential genes promoting antiviral activity.

引用

页码：77 / 85

页数：9

共 29 条

[1]

[Anonymous], 2012, Molecular Cloning: A Laboratory Manual

[2] GENETIC AND STRUCTURAL CHARACTERIZATION OF THE AVIRULENCE GENE AVRBS3 FROM XANTHOMONAS-CAMPESTRIS PV VESICATORIA [J].