Polarization of chemokine receptors to the leading edge during lymphocyte chemotaxis

被引:175
作者
Nieto, M
Frade, JMR
Sancho, D
Mellado, M
Martinez, C
SanchezMadrid, F
机构
[1] UNIV AUTONOMA MADRID,HOSP PRINCESA,SERV IMMUNOL,E-28006 MADRID,SPAIN
[2] UNIV AUTONOMA MADRID,CSIC,CTR NACL BIOTECNOL,DEPT IMMUNOL & ONCOL,E-28049 MADRID,SPAIN
关键词
D O I
10.1084/jem.186.1.153
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Leukocyte migration in response to cell attractant gradients or chemotaxis is a key phenomenon both in cell movement and in the inflammatory response. Chemokines are quite likely to be the key molecules directing migration of leukocytes that involve cell polarization with generation of specialized cell compartments. The precise mechanism of leukocyte chemoattraction is not known, however. In this study, we demonstrate that the CC chemokine receptors CCR2 and CCR5, but not cytokine receptors such as interleukin (IL)-2R alpha, IL-2R beta, tumor necrosis factor receptor 1, or transforming growth factor beta R, are redistributed to a pole in T cells that are migrating in response to chemokines. Immunofluorescence and confocal microscopy studies show that the chemokine receptors concentrate at the leading edge of the cell on the flattened cell-substratum contact area, induced specifically by the signals that trigger cell polarization. The redistribution of chemokine receptors is blocked by pertussis toxin and is dependent on cell adhesion through integrin receptors, which mediate cell migration. Chemokine receptor expression on the leading edge of migrating polarized lymphocytes appears to act as a sensor mechanism for the directed migration of leukocytes through a chemoattractant gradient.
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页码:153 / 158
页数:6
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