Mammalian Heat Shock Factor 1 Is Essential for Oocyte Meiosis and Directly Regulates Hsp90α Expression

被引:83
作者
Metchat, Aicha [1 ]
Akerfelt, Malin [2 ]
Bierkamp, Christiane [1 ]
Delsinne, Virginie [3 ]
Sistonen, Lea [2 ]
Alexandre, Henri [3 ]
Christians, Elisabeth S. [1 ]
机构
[1] Univ Toulouse, 4R3B3, Ctr Dev Biol, UPS,UMR5547, F-31062 Toulouse, France
[2] Abo Akad Univ, Univ Turku, Turku Ctr Biotechnol, Dept Biol, FIN-20520 Turku, Finland
[3] Univ Mons, Dept Biol, Fac Med Pharm, B-7000 Mons, Belgium
关键词
TARGETED GENE DISRUPTION; MOUSE OOCYTES; TRANSCRIPTION FACTOR-1; DOWN-REGULATION; CYCLIN B1; MICE; HSF1; MATURATION; THERMOTOLERANCE; TRANSITION;
D O I
10.1074/jbc.M808819200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Heat shock transcription factor 1 (HSF1) is the main regulator of the stress response that triggers the transcription of several genes encoding heat shock proteins (Hsps). Hsps act as molecular chaperones involved in protein folding, stability, and trafficking. HSF1 is highly expressed in oocytes and Hsf1 knock-out in mice revealed that in the absence of stress this factor plays an important role in female reproduction. We previously reported that Hsf1(-/-) females produce oocytes but no viable embryos. Consequently, we asked whether oocytes require HSF1 to regulate a particular set of Hsps necessary for them to develop. We find that Hsp90 alpha (Hspaa1) is the major HSF1-dependent chaperone inasmuch as Hsf1 knock-out resulted in Hsp90-depleted oocytes. These oocytes exhibited delayed germinal vesicle breakdown (or G(2)/M transition), partial meiosis I block, and defective asymmetrical division. To probe the role of Hsp90 alpha in this meiotic syndrome, we analyzed meiotic maturation in wildtype oocytes treated with a specific inhibitor of Hsp90, 17-allylamino-17-demethoxy-geldanamycin, and observed similar defects. At the molecular level we showed that, together with these developmental anomalies, CDK1 and MAPK, key meiotic kinases, were significantly disturbed. Thus, our data demonstrate that HSF1 is a maternal transcription factor essential for normal progression of meiosis.
引用
收藏
页码:9521 / 9528
页数:8
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