Rapid direct detection of the major fish allergen, parvalbumin, by selected MS/MS ion monitoring mass spectrometry

被引：99

作者：

Carrera, Monica ^{[1
,2
]}

Canas, Benito ^{[3
]}

Gallardo, Jose M. ^{[2
]}

机构：

[1] ETH, IMSB, CH-8093 Zurich, Switzerland

[2] CSIC, Marine Res Inst IIM, Vigo, Pontevedra, Spain

[3] Univ Complutense Madrid, Madrid, Spain

来源：

JOURNAL OF PROTEOMICS | 2012年 / 75卷 / 11期

关键词：

Allergen; Proteomics; Selected MS/MS Ion Monitoring (SMIM); High Intensity Focused Ultrasound; Mass spectrometry; Parvalburnin; Food; CALCIUM-BINDING; PROTEIN IDENTIFICATION; PROTEOMICS; DIGESTION; CHALLENGE; PEPTIDES; CLONING; CODFISH;

D O I：

10.1016/j.jprot.2012.03.030

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

Parvalbumins beta (beta-PRVBs) are considered the major fish allergens. A new strategy for the rapid and direct detection of these allergens in any foodstuff is presented in this work. The proposed methodology is based on the purification of beta-PRVBs by treatment with heat, the use of accelerated in-solution trypsin digestion under an ultrasonic field provided by High-Intensity Focused Ultrasound (HIFU) and the monitoring of only nineteen beta-PRVB peptide biomarkers by Selected MS/MS Ion Monitoring (SMIM) in a linear ion trap (LIT) mass spectrometer. The present strategy allows the direct detection of the presence of fish beta-PRVBs in any food product in less than 2 hours. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：3211 / 3220

页数：10

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