Use of a rapid reverse-transcription recombinase aided amplification assay for respiratory syncytial virus detection

被引：92

作者：

Chen, Chen ^{[1
]}

Li, Xin-na ^{[1
]}

Li, Gui-xia ^{[2
]}

Zhao, Li ^{[1
,2
]}

Duan, Su-xia ^{[1
,2
]}

Yan, Teng-fei ^{[1
,3
]}

Feng, Zhi-shan ^{[2
]}

Ma, Xue-jun ^{[1
]}

机构：

[1] Natl Inst Viral Dis Control & Prevent, Chinese Ctr Dis Control & Prevent, Key Lab Med Virol Natl Hlth & Family Planning Com, Beijing, Peoples R China

[2] Childrens Hosp Hebei Prov, Pediat Res Inst, Shijiazhuang 050031, Hebei, Peoples R China

[3] First Hosp Shijiazhuang, Myasthenia Gravis Res Inst, Shijiazhuang 050011, Hebei, Peoples R China

来源：

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE | 2018年 / 90卷 / 02期

关键词：

Respiratory syncytial virus; Detection; Subgroup; RT-RAA; MEDIATED ISOTHERMAL AMPLIFICATION; REAL-TIME PCR; VIRAL CULTURE; INFECTION; ADULTS; DIAGNOSIS; PREVALENCE; COMMUNITY; ILLNESS; SEASONS;

D O I：

10.1016/j.diagmicrobio.2017.10.005

中图分类号：

R51 [传染病];

学科分类号：

100201 [内科学];

摘要：

In this study, a rapid reverse-transcription recombinase aided amplification (RT-RAA) assay was developed to detect respiratory syncytial virus (RSV) subgroups A and B, respectively. The reaction was performed at 39 C in less than 30 min. The analytical sensitivities of RSVA and RSVB at 95% probability by probit regression analysis were 38copies per reaction and 35 copies per reaction, respectively, and no cross reactions with other related respiratory viruses were observed. The RT-RAA assay was further utilized to detect and subgroup 306 clinical specimens and the results showed that 79(25.82%, 79/306) samples were positive for RSV, of those 16(20.25%, 16/79) were identified as RSVA and 63(79.75%, 63/79) were RSVB, which is completely consistent with the results obtained by RSV RT-qPCR assay. In conclusion, the developed RAA assay will be of benefit as a faster, sensitive and specific alternative tool for detection of RSV. (C) 2017 Elsevier Inc. All rights reserved.

引用

页码：90 / 95

页数：6

共 35 条

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