Optical recording of cortical activity after in vitro perfusion of cerebral arteries with a voltage-sensitive dye

被引:31
作者
de Curtis, M
Takashima, I
Iijima, T
机构
[1] Ist Nazl Neurol, Dept Expt Neurophysiol, I-20133 Milan, Italy
[2] Sect Cellular & Mol Neurosci, Electrotech Lab, Tsukuba, Ibaraki 305, Japan
关键词
entorhinal cortex; isolated guinea pig brain; optical imaging; piriform cortex; voltage-sensitive dyes;
D O I
10.1016/S0006-8993(99)01712-6
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Cortical neuronal architecture and connectivity can be analyzed with high-resolution optical imaging after staining the in vitro isolated guinea pig brain preparation by circulating the voltage-sensitive dye RH795 via the arterial system. To establish this new technique, electrical field potentials evoked in the piriform and entorhinal cortices by lateral olfactory tract stimulation were correlated to the optical signal. The depth analysis of the optical response was performed by evaluating the contribution of the mano- and poly-synaptic components of the signal generated in different layers after applying a pair-pulse stimulation protocol. The tangential propagation of neuronal activity in olfactory cortices was evaluated by gathering several 4.2 x 4.2 mm images recorded from adjacent cortical areas. The real-time optical imaging technique applied to the isolated guinea pig brain can be successfully utilized to study the integrative properties of cortical neurons ensembles. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:314 / 319
页数:6
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