Early steps in the biosynthesis of NAD in Arabidopsis start with aspartate and occur in the plastid

被引:156
作者
Katoh, Akira
Uenohara, Kazuya
Akita, Mitsuru
Hashimoto, Takashi [1 ]
机构
[1] Nara Inst Sci & Technol, Grad Sch Biol Sci, Nara 6300192, Japan
[2] Ehime Univ, Fac Agr & Venture Business Lab, Matsuyama, Ehime 7908577, Japan
关键词
D O I
10.1104/pp.106.081091
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
NAD is a ubiquitous coenzyme involved in oxidation-reduction reactions and is synthesized by way of quinolinate. Animals and some bacteria synthesize quinolinate from tryptophan, whereas other bacteria synthesize quinolinate from aspartate ( Asp) using L-Asp oxidase and quinolinate synthase. We show here that Arabidopsis ( Arabidopsis thaliana) uses the Asp-to-quinolinate pathway. The Arabidopsis L-Asp oxidase or quinolinate synthase gene complemented the Escherichia coli mutant defective in the corresponding gene, and T-DNA-based disruption of either of these genes, as well as of the gene coding for the enzyme quinolinate phosphoribosyltransferase, was embryo lethal. An analysis of functional green fluorescent protein-fused constructs and in vitro assays of uptake into isolated chloroplasts demonstrated that these three enzymes are located in the plastid.
引用
收藏
页码:851 / 857
页数:7
相关论文
共 31 条
[1]   De novo and salvage biosynthetic pathways of pyridine nucleotides and nicotinic acid conjugates in cultured plant cells [J].
Ashihara, H ;
Stasolla, C ;
Yin, YL ;
Loukanina, N ;
Thorpe, TA .
PLANT SCIENCE, 2005, 169 (01) :107-114
[2]   Proteome analysis of tobacco bright yellow-2 (BY-2) cell culture plastids as a model for undifferentiated heterotrophic plastids [J].
Baginsky, S ;
Siddique, A ;
Gruissem, W .
JOURNAL OF PROTEOME RESEARCH, 2004, 3 (06) :1128-1137
[3]  
Bruce BD, 1994, PLANT MOL BIOL MAN J, VJ1, P1
[4]   Increase in activity during calorie restriction requires Sirt1 [J].
Chen, D ;
Steele, AD ;
Lindquist, S ;
Guarente, L .
SCIENCE, 2005, 310 (5754) :1641-1641
[5]   Engineered GFP as a vital reporter in plants [J].
Chiu, WL ;
Niwa, Y ;
Zeng, W ;
Hirano, T ;
Kobayashi, H ;
Sheen, J .
CURRENT BIOLOGY, 1996, 6 (03) :325-330
[6]   Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].
Clough, SJ ;
Bent, AF .
PLANT JOURNAL, 1998, 16 (06) :735-743
[7]  
CORUZZI G, 2000, BIOCH MOL BIOL PLANT, P1250
[8]  
DAWSON RF, 1958, CHEM IND-LONDON, P100
[9]   BIOSYNTHESIS OF THE PYRIDINE RING OF NICOTINE [J].
DAWSON, RF ;
CHRISTMAN, DR ;
ANDERSON, RC ;
SOLT, ML ;
DADAMO, AF ;
WEISS, U .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1956, 78 (11) :2645-2646
[10]   Dual targeting is the rule for organellar aminoacyl-tRNA synthetases in Arabidopsis thaliana [J].
Duchêne, AM ;
Giritch, A ;
Hoffmann, B ;
Cognat, V ;
Lancelin, D ;
Peeters, NM ;
Zaepfel, M ;
Maréchal-Drouard, L ;
Small, ID .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (45) :16484-16489