A Plasma Membrane Receptor Kinase, GHR1, Mediates Abscisic Acid- and Hydrogen Peroxide-Regulated Stomatal Movement in Arabidopsis

被引：328

作者：

Hua, Deping ^{[1
]}

Wang, Cun ^{[1
]}

He, Junna ^{[1
]}

Liao, Hui ^{[1
]}

Duan, Ying ^{[1
]}

Zhu, Ziqiang ^{[1
]}

Guo, Yan ^{[1
]}

Chen, Zhizhong ^{[1
]}

Gong, Zhizhong ^{[1
,2
]}

机构：

[1] China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China

[2] Natl Ctr Plant Gene Res, Beijing 100193, Peoples R China

来源：

PLANT CELL | 2012年 / 24卷 / 06期

基金：

中国国家自然科学基金;

关键词：

PROTEIN PHOSPHATASE 2C; OXYGEN SPECIES PRODUCTION; ANION CHANNEL SLAC1; SIGNAL-TRANSDUCTION; GUARD-CELLS; ABA; CLOSURE; ABI1; ACTIVATION; RESISTANCE;

D O I：

10.1105/tpc.112.100107

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The plant hormone abscisic acid (ABA) regulates stomatal movement under drought stress, and this regulation requires hydrogen peroxide (H2O2). We isolated GUARD CELL HYDROGEN PEROXIDE-RESISTANT1 (GHR1), which encodes a receptor-like kinase localized on the plasma membrane in Arabidopsis thaliana. ghr1 mutants were defective ABA and H2O2 induction of stomatal closure. Genetic analysis indicates that GHR1 is a critical early component in ABA signaling. The ghr1 mutation impaired ABA- and H2O2-regulated activation of S-type anion currents in guard cells. Furthermore, GHR1 physically interacted with, phosphorylated, and activated the S-type anion channel SLOW ANION CHANNEL-ASSOCIATED1 when coexpressed in Xenopus laevis oocytes, and this activation was inhibited by ABA-INSENSITIVE2 (ABI2) but not ABI1. Our study identifies a critical component in ABA and H2O2 signaling that is involved in stomatal movement and resolves a long-standing mystery about the differential functions of ABI1 and ABI2 in this process.

引用

页码：2546 / 2561

页数：16

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