Cloning and sequence analysis of the gene encoding 3-hexulose-6-phosphate synthase from the methylotrophic bacterium, Methylomonas aminofaciens 77a, and its expression in Escherichia coli

被引：25

作者：

Yanase, H ^{[1
]}

Ikeyama, K ^{[1
]}

Mitsui, R ^{[1
]}

Ra, S ^{[1
]}

Kita, K ^{[1
]}

Sakai, Y ^{[1
]}

Kato, N ^{[1
]}

机构：

[1] KYOTO UNIV,FAC AGR,DEPT AGR CHEM,KYOTO 60601,JAPAN

来源：

FEMS MICROBIOLOGY LETTERS | 1996年 / 135卷 / 2-3期

关键词：

hexulose phosphate synthase gene; methylotroph; ribulose monophosphate cycle; Methylomonas aminofaciens;

D O I：

10.1016/0378-1097(95)00450-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A DNA fragment of 550 bp was specifically amplified by PCR with primers based on the N-terminal sequence of the purified 3-hexulose-6-phosphate synthase from Methylomonas aminofaciens 77a and on that of a,lysyl endopeptidase-derived peptide. Using this PCR product as a probe, a gene coding for 3-hexulose-6-phosphate synthase in M. aminofaciens 77a chromosomal DNA was cloned in Escherichia coli JM109. Sequencing analysis revealed that the gene encoding 3-hexulose-6-phosphate synthase contained a 624-bp open reading frame, encoding a protein composed of 208 amino acid residues with a calculated relative molecular mass of 21224.

引用

页码：201 / 205

页数：5

共 10 条

[1]

ARFMAN N, 1993, THESIS U GRONINGEN

[2] 3-HEXULOSE-PHOSPHATE SYNTHASE FROM METHYLOMONAS-AMINOFACIENS 77A - PURIFICATION, PROPERTIES AND KINETICS [J].