Effect of short-term treatment with a monoclonal antibody to P-selectin on balloon catheter-induced: Intimal hyperplasia, re-endothelialization, and attenuation of endothelial-dependent relaxation

被引:5
作者
Akers, DL
Lefer, DJ
Chen, IL
Wilkens, RG
Rice, J
Aurora, H
Osgood, TA
Bedi, B
Tenaglia, AN
Buda, AJ
Kadowitz, PJ
McNamara, DB
机构
[1] TULANE UNIV, SCH MED, DEPT PHARMACOL SL83, NEW ORLEANS, LA 70112 USA
[2] TULANE UNIV, SCH MED, DEPT SURG, NEW ORLEANS, LA 70112 USA
[3] TULANE UNIV, SCH MED, DEPT MED, NEW ORLEANS, LA 70112 USA
[4] TULANE UNIV, SCH MED, DEPT ANAT, NEW ORLEANS, LA 70112 USA
[5] TULANE UNIV, SCH TROP HLTH & MED, NEW ORLEANS, LA 70112 USA
关键词
endothelium; polymorphonuclear leukocyte; nitric oxide; intimal hyperplasia; P-selectin;
D O I
10.1023/A:1006858424790
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The effects of an anti-P-selectin monoclonal antibody (MAb, PB1.3; Cytel Corporation) on neoendothelialization; neoendothelial function, as evidenced by acetylcholine-induced relaxation (nitric oxide formation); and intimal hyperplasia following embolectomy catheter-induced injury to the rabbit thoracic aorta were investigated. Catheter injury was induced in two groups of New Zealand White rabbits. One group received no treatment, while the second group received short-term treatment with the MAb (i.p., immediately before and 12 h after induction of catheter injury), A third group underwent a sham operation and served as uninjured controls. Following sacrifice at 2 weeks after injury, aortic rings were assessed for degree of intimal hyperplasia, neoendothelial morphology (scanning electron microscopy), and acetylcholine-induced relaxation. Aortic tissue from catheter-injured animals that received treatment exhibited improved neoendothelial morphology, as compared with tissue from untreated but catheterized animals; however, no statistically significant attenuation of the hyperplastic response or improvement in the attenuated neoendothelial-dependent acetylcholine-induced relaxant response that is characteristic of neoendothelium that forms after catheter denudation was observed. These data suggest that short term attenuation of P-selectin-mediated poly morphonuclear leukocyte (PMN)endothelium, PMN/platelet interactions, and/or thrombin formation beneficially affects neoendothelialization of the vascular wall following balloon catheter-induced injury.
引用
收藏
页码:13 / 20
页数:8
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