Stromal cells from the adipose tissue-derived stromal vascular fraction and culture expanded adipose tissue-derived stromal/stem cells: a joint statement of the International Federation for Adipose Therapeutics and Science (IFATS) and the International Society for Cellular Therapy (ISCT)

被引：1376

作者：

Bourin, Philippe ^{[1
]}

Bunnell, Bruce A. ^{[2
]}

Casteilla, Louis ^{[3
]}

Dominici, Massimo ^{[4
]}

Katz, Adam J. ^{[5
]}

March, Keith L. ^{[6
]}

Redl, Heinz ^{[7
]}

Rubin, J. Peter ^{[8
]}

Yoshimura, Kotaro ^{[9
]}

Gimble, Jeffrey M. ^{[10
]}

机构：

[1] CSA21, F-31100 Toulouse, France

[2] Tulane Univ, Sch Med, Dept Pharmacol, Ctr Stem Cell Res & Regenerat Med, New Orleans, LA 70112 USA

[3] CNRS, StromaLab, UMR UPS, Inserm,EFS 5273,U1031, Toulouse, France

[4] Univ Hosp Modena & Reggio Emilia, Lab Cell Biol & Adv Canc Therapies, Dept Med & Surg Sci Adults & Children, Modena, Italy

[5] Univ Florida, Dept Surg, Div Plast & Reconstruct Surg, Gainesville, FL USA

[6] Indiana Univ Sch Med, Indiana Ctr Vasc Biol & Med, Indianapolis, IN USA

[7] Austrian Cluster Tissue Regenerat, AUVA Res Ctr, Ludwig Boltzmann Inst Expt & Clin Traumatol, Vienna, Austria

[8] Univ Pittsburgh, Dept Plast Surg, Pittsburgh, PA USA

[9] Univ Tokyo, Sch Med, Dept Plast Surg, Tokyo 113, Japan

[10] Louisiana State Univ Syst, Pennington Biomed Res Ctr, Stem Cell Biol Lab, Baton Rouge, LA USA

来源：

CYTOTHERAPY | 2013年 / 15卷 / 06期

关键词：

adipose-derived stromal/stem cells; adipose tissue; characterization; function; phenotype; stromal vascular fraction; HUMAN-BONE-MARROW; STEM-CELLS; HEMATOPOIETIC STEM; IN-VITRO; FEATURES; SURFACE;

D O I：

10.1016/j.jcyt.2013.02.006

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Background aims. Adipose tissue is a rich and very convenient source of cells for regenerative medicine therapeutic approaches. However, a characterization of the population of adipose-derived stromal and stem cells (ASCs) with the greatest therapeutic potential remains unclear. Under the authority of International Federation of Adipose Therapeutics and International Society for Cellular Therapy, this paper sets out to establish minimal definitions of stromal cells both as uncultured stromal vascular fraction (SVF) and as an adherent stromal/stem cells population. Methods. Phenotypic and functional criteria for the identification of adipose-derived cells were drawn from the literature. Results. In the SVF, cells are identified phenotypically by the following markers: CD45-CD235a-CD31-CD34+. Added value may be provided by both a viability marker and the following surface antigens: CD13, CD73, CD90 and CD105. The fibroblastoid colony-forming unit assay permits the evaluation of progenitor frequency in the SVF population. In culture, ASCs retain markers in common with other mesenchymal stromal/stem cells (MSCs), including CD90, CD73, CD 105, and CD44 and remain negative for CD45 and CD31. They can be distinguished from bone-marrow-derived MSCs by their positivity for CD36 and negativity for CD106. The CFU-F assay is recommended to calculate population doublings capacity of ASCs. The adipocytic, chondroblastic and osteoblastic differentiation assays serve to complete the cell identification and potency assessment in conjunction with a quantitative evaluation of the differentiation either biochemically or by reverse transcription polymerase chain reaction. Conclusions. The goal of this paper is to provide initial guidance for the scientific community working with adipose-derived cells and to facilitate development of international standards based on reproducible parameters.

引用

页码：641 / 648

页数：8

共 32 条

[1] In vitro and multicolor phenotypic characterization of cell subpopulations identified in fresh human adipose tissue stromal vascular fraction and in the derived mesenchymal stem cells [J].