A highly sensitive HPLC method to discriminate enantiomeric amino deoxy sugars based on the derivatization with (S)-(+)-TBMB carboxylic acid

被引：12

作者：

Bai, C

Nishida, Y

Ohrui, H

Meguro, H

机构：

[1] Dept. of Appl. Biological Chemistry, Faculty of Agriculture, Tohoku University, Sendai 981

来源：

JOURNAL OF CARBOHYDRATE CHEMISTRY | 1996年 / 15卷 / 02期

关键词：

D O I：

10.1080/07328309608005440

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A picomole determination of the absolute configurations of amino deoxy sugars was demonstrated through the derivatization at the anomeric carbon with a fluorescent chiral reagent, (S)-(+)-TBMB carboxylic acid, followed by the HPLC separations of the derived diastereomeric isomers. Anew chemical procedure was developed to convert amino deoxy sugars into per-NH,O-acetylated glycosyl chlorides prior to derivatization with the reagent.

引用

页码：217 / 230

页数：14

共 16 条

[1]

Brimacombe J.S., 1967, CARBOHYD RES, V3, P318

[2]

CONCHIE J, 1963, METHODS CARBOHYDRATE, V2, P332

[3]

CRAMER FD, 1962, METHODS CARBOHYDRATE, V1, P243

[4] KANAMYCIN .4. THE STRUCTURE OF KANAMYCIN [J].