HPLC method for cyst(e)ine and methionine in infant formulas

被引：8

作者：

Toran, AA

Barbera, R

Farre, R

Lagarda, MJ

Lopez, JC

机构：

[1] Dept. of Nutrition Chemistry, Univ. of Valencia, 46100-Burjassot, Avda. Vicente Andres Estelles s/n

来源：

JOURNAL OF FOOD SCIENCE | 1996年 / 61卷 / 06期

关键词：

HPLC; infant formula; milk; cyst(e)ine; methionine;

D O I：

10.1111/j.1365-2621.1996.tb10946.x

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Cyst(e)ine and methionine were converted into cysteic acid and methionine sulfone by oxidizing with performic acid. The oxidized samples were then subjected to acid hydrolysis (6N HCl, 105-110 degrees C/24 hr). After derivatization with phenylisothiocyanate, reverse phase HPLC separation was carried out al 48 degrees C and with UV detection. Different gradients and pH values in the eluent were assayed to determine the best resolution. Analytical parameters, detection and quantification limits, linearity, precision and accuracy, were determined. The method was reliable and accurate for measuring cyst(e)ine and methionine in infant formulae.

引用

页码：1132 / &

页数：5

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