Protein NMR in biomedical research

被引：64

作者：

Jahnke, W ^{[1
]}

Widmer, H ^{[1
]}

机构：

[1] Novartis Pharma AG, Novartis Inst Biomed Res, CH-4002 Basel, Switzerland

来源：

CELLULAR AND MOLECULAR LIFE SCIENCES | 2004年 / 61卷 / 05期

关键词：

fragment-based screening; drug discovery; NMR screening; SAR-by-NMR; structure determination; high throughput screening; hit validation;

D O I：

10.1007/s00018-003-3382-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nuclear magnetic resonance (NMR) spectroscopy is a versatile biophysical technique with wide applicability in drug discovery research, particularly for the detection and characterization of molecular interactions. This review highlights in a comprehensive manner the aspects of biomolecular NMR which are most beneficial for pharmaceutical research and presents them as contributions to the different stages of a drug discovery program: target selection, assay development, lead generation and lead optimization. Emphasis is put on the concept of the particular NMR application, rather than on technical details, and on recent examples. Finally, an appendix of frequently asked questions is given.

引用

页码：580 / 599

页数：20

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