Genomic organization and expression of the human MSH3 gene

被引:49
作者
Watanabe, A [1 ]
Ikejima, M [1 ]
Suzuki, N [1 ]
Shimada, T [1 ]
机构
[1] NIPPON MED COLL,DEPT BIOCHEM & MOLEC BIOL,BUNKYO KU,TOKYO 113,JAPAN
关键词
D O I
10.1006/geno.1996.0053
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We have studied the expression and genomic organization of the human MSH3 gene, which encodes a human homologue of the bacterial DNA mismatch repair protein MutS. This gene is located upstream of the dihydrofolate reductase (DHFR) gene. Northern analysis has demonstrated that the hMSH3 gene is expressed in a variety of human tissues at low levels, like the DHFR gene. Characterization of cosmid clones has shown that the hMSH3 gene consists of 24 exons spanning at least 160 kb. All exon-intron junction sequences match the classical GT/AG rule, except that intron 6 has AT and AA at the ends. Two major transcripts of 5.0 and 3.8 kb have been shown to be derived from the differential use of two polyadenylation sites. Elucidation of the complete genomic organization and the nucleotide sequences of the introns of the hMSH3 gene should be useful for studying the function of this gene and the possible involvement of specific mutations of the hMSH3 gene in some diseases. (C) 1996 Academic Press, Inc.
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页码:311 / 318
页数:8
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