Endogenous oxidative DNA base modifications analysed with repair enzymes and GC/MS technique

被引：47

作者：

Jaruga, Pawel ^{[1
]}

Speina, Elzbieta ^{[2
]}

Gackowski, Daniel ^{[1
]}

Tudek, Barbara ^{[2
]}

Olinski, Ryszard ^{[1
]}

机构：

[1] L Rydygier Med Univ, Dept Clin Biochem, PL-85092 Bydgoszcz, Poland

[2] Polish Acad Sci, Inst Biochem & Biophys, Warsaw, Poland

来源：

NUCLEIC ACIDS RESEARCH | 2000年 / 28卷 / 06期

关键词：

D O I：

10.1093/nar/28.6.e16

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

GC/MS technique was used to identify endogenous levels of oxidatively modified DNA bases. To avoid possible artefact formation we used Fpg and Endo III endonucleases instead of acid hydrolysis to liberate the base products from unmodified DNA samples. Several different DNA preparations were used: (i) commercial calf thymus DNA, (ii) DNA isolated from rat liver, (iii) DNA isolated from human lymphocytes and (iv) nuclei isolated from rat liver. In all DNA samples used in our assays the most efficiently removed bases by Fpg protein are FapyG and FapyA although 8-oxoG was also detected in all preparations. The amount of 8-oxoG in human lymphocytes and in rat liver DNA was 3 and 2 per 10(7) bases, respectively. It is reasonable to postulate that the presented method is one of the techniques which should be used to reveal the enigma of endogenous, oxidative DNA damage.

引用

页数：4

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