Wnt/β-catenin signaling regulates neuronal differentiation of mesenchymal stem cells

被引：51

作者：

Yu, Qin ^{[1
]}

Liu, Lizhen ^{[2
]}

Duan, Yanping ^{[1
]}

Wang, Yan ^{[3
]}

Xuan, Xiaobo ^{[3
]}

Zhou, Liping ^{[1
]}

Liu, Wei ^{[1
]}

机构：

[1] Zhejiang Chinese Med Univ, Inst Bioengn, Hangzhou 310053, Zhejiang, Peoples R China

[2] Zhejiang Univ, Sch Med, Affiliated Hosp 1, Bone Marrow Transplantat Ctr, Hangzhou 310003, Zhejiang, Peoples R China

[3] Zhejiang Chinese Med Univ, Affiliated Hosp 1, Hangzhou 310053, Zhejiang, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2013年 / 439卷 / 02期

关键词：

Mesenchymal stem cells; Wnt/beta-catenin signaling; Neuronal differentiation; Wnt-3a; MARROW STROMAL CELLS; BONE-MARROW; MECHANISMS; WNT-3A; TRANSPLANTATION; PROGENITORS; EXPRESSION; INDUCTION; INJURY; MICE;

D O I：

10.1016/j.bbrc.2013.08.030

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Mesenchymal stem cells (MSCs) have been demonstrated to be able to differentiate into neuron-like cells, but the precise mechanisms controlling this process are unclear. Using neuron-specific enolase (NSE) and nestin as neuronal markers, we examined the role of Wnt/beta-catenin signaling in MSC neuronal differentiation in present study. The results indicated that the expression of beta-catenin increased markedly during the neuronal differentiation of MSCs. Blocking Wnt signaling by treating MSCs with beta-catenin siRNA could decrease the differentiation of MSCs into neuron-like cells and up-regulation of Wnt signaling by treating MSCs with Wnt-3a could promote neuronal differentiation of MSCs. Above results suggest that Wnt/beta-catenin signaling may play a pivotal role in neuronal differentiation of MSCs. Our data broaden the knowledge of molecular mechanisms involved in the neuronal differentiation of MSCs and provide a potential target for directing differentiation of MSCs for clinical application. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：297 / 302

页数：6

共 25 条

[1]

Bone marrow-derived mesenchymal stem cells promote neuronal networks with functional synaptic transmission after transplantation into mice with neurodegeneration [J].