Demethylation-induced developmental pleiotropy in Arabidopsis

被引：393

作者：

Ronemus, MJ ^{[1
]}

Galbiati, M ^{[1
]}

Ticknor, C ^{[1
]}

Chen, JC ^{[1
]}

Dellaporta, SL ^{[1
]}

机构：

[1] YALE UNIV,DEPT BIOL,NEW HAVEN,CT 06520

来源：

SCIENCE | 1996年 / 273卷 / 5275期

关键词：

D O I：

10.1126/science.273.5275.654

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The function of DNA methylation in higher plants was investigated by expression of a complementary DNA encoding a cytosine methyltransferase (MET1) from Arabidopsis thaliana as an antisense RNA in transgenic plants. This expression resulted in a 34 to 71 percent reduction in total genomic cytosine methylation. Loss of methylation was observed in both repetitive DNA and single-copy gene sequences. Developmental effects included altered heterochrony, changes in meristem identity and organ number, and female sterility. Cytosine demethylation prolonged both vegetative and reproductive phases of development. These findings implicate DNA methylation in establishing or maintaining epigenetic developmental states in the meristem.

引用

页码：654 / 657

页数：4

共 33 条

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