Sensitivity and Signal Complexity as a Function of the Number of Ion Channels in a Stochastic Sensor

被引：50

作者：

Ervin, Eric N. ^{[1
]}

White, Ryan J. ^{[1
]}

White, Henry S. ^{[1
]}

机构：

[1] Univ Utah, Dept Chem, Salt Lake City, UT 84112 USA

来源：

ANALYTICAL CHEMISTRY | 2009年 / 81卷 / 02期

关键词：

GLASS NANOPORE MEMBRANES; STAPHYLOCOCCAL ALPHA-HEMOLYSIN; MOLECULAR ADAPTER; PROTEIN PORES;

D O I：

10.1021/ac801104v

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Alternating current, phase-sensitive stochastic detection using between 1 and 26 alpha-hemolysin ion channels reconstituted in a lipid bilayer, suspended over a 160-nm-radius orifice glass nanopore, is reported. As predicted by the binomial distribution, simultaneous analyte detection at large numbers of channels is effectively zero, independent of the number of ion channels. The results indicate that alpha HL channels are noninteracting and that significant gains in sensitivity are possible without sacrificing the simplicity of single-molecule detection strategies.

引用

页码：533 / 537

页数：5

共 21 条

[1]

Alvarez O., 1986, ION CHANNEL RECONSTI, P115

[2] Toward single molecule DNA sequencing:: Direct identification of ribonucleoside and deoxyribonucleoside 5′-monophosphates by using an engineered protein nanopore equipped with a molecular adapter [J].