Comparative study in vivo and in vitro of uniformly C-14-labelled and I-125-labelled recombinant fibroblast growth factor 2

Recombinant bovine fibroblast growth factor (FGF2), uniformly labelled with C-14 ([C-14]FGF2), was purified and showed to be highly stable and to retain full biological activity. Orsan distribution of [C-14]FGF2 after intravenous injection of young rats was assessed by autoradiography of whole body sections and compared with those obtained with [I-125]iodinated FGF2 (I-125-FGF2). Thyroid, stomach, intestine, bladder and skin were radioactive ly labelled only in the case of I-125-FGF2. This tissue-labelling is artefactual, probably due to free iodide binding not observed when using [C-14]FGF2. High-resolution autoradiography showed a complex tissue distribution of [C-14]FGF2 in kidney and adrenal organs. Incubation of frozen eye sections with [C-14]FGF2 showed a specific and high-resolution labelling pattern of ocular tissues. After cellular internalization, [C-14]FGF2 was processed into five distinct polypeptides of 16, 14, 8, 7, and 5.5 kDa. The 14-kDa and 7-kDa polypeptides are novel catabolic fragments not detected With radioiodinated FGF,. In terms of stability, tissue distribution specificity, and autoradiographic resolution, [C-14]FGF2 proved to have more advantages than I-125-FGF2 for pharmacokinetic and catabolism studies.