Evolutionary dynamics of cucumber mosaic virus satellite RNA during natural epidemics in Italy

被引:38
作者
Grieco, F
Lanave, C
Gallitelli, D
机构
[1] UNIV BARI, DIPARTIMENTO PROTEZ PIANTE, I-70126 BARI, ITALY
[2] CNR, CTR STUDIO VIRUS & VIROSI COLTURE MEDITERRANEE, I-70126 BARI, ITALY
[3] CNR, CTR STUDIO MITOCONDRI & METAB ENERGET, I-70126 BARI, ITALY
关键词
D O I
10.1006/viro.1996.8426
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The evolutionary dynamics of 22 variants of cucumber mosaic virus satellite RNA (CMV satRNA) isolated in Italy during virus epidemics from 1988 to 1993 were investigated on the basis of their primary structure and biological properties. Most of the variants were amplified from total nucleic acid preparations extracted from field-infected plants, thus representing wild isolates of CMV satRNA. Eleven variants were associated with subgroup II CMV strains, 10 with subgroup I and 1 with a mixed infection by both strains. When inoculated onto tomato seedlings, the variants induced the phenotype (necrogenic or ameliorative) predicted by their nucleotide sequence. Phylogenetic relationships between the satRNA variants were determined using the stationary Markov model, a stochastic model for evolution. For each satRNA, the Markov analysis gave a good correlation between position in the phylogenetic tree and biological properties. The variants with ameliorative and necrogenic phenotypes in tomato followed two different evolutionary dynamics in nature. Tfn-satRNA, a 390-nt-long molecule, followed a third type of evolutionary dynamic far apart from that of the shorter satRNA molecules (i.e., those in the 334- to 340-nt-length class). Average values of the mean constant rate of nucleotide substitutions/site (K-subs/site) indicated that in nature the variants tend to keep their heterogeneity unchanged from one epidemic episode to the other, even if the outbreaks occur in places very far from each other. This seems to be in agreement with the proposed maintenance of a functional molecular structure as a constraint to CMV satRNA evolution. (C) 1997 Academic Press.
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页码:166 / 174
页数:9
相关论文
共 43 条
[1]   GENETIC-VARIABILITY AND EVOLUTION OF THE SATELLITE RNA OF CUCUMBER MOSAIC-VIRUS DURING NATURAL EPIDEMICS [J].
ARANDA, MA ;
FRAILE, A ;
GARCIAARENAL, F .
JOURNAL OF VIROLOGY, 1993, 67 (10) :5896-5901
[2]   USE OF THE POLYMERASE CHAIN-REACTION AND SANDWICH-HYBRIDIZATION FOR DETECTING ARTICHOKE MOTTLED CRINKLE TOMBUSVIRUS IN ARTICHOKE [J].
BARBAROSSA, L ;
GRIECO, F ;
IOSCO, P ;
GALLITELLI, D .
JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT, 1994, 140 (03) :201-208
[3]  
Cillo Cillo F. F., ACTA HORTIC, V379 379, P369, DOI [10.17660/ActaHortic.1994.376.51 10.17660/ActaHortic.1994.376.51, DOI 10.17660/ACTAHORTIC.1994.376.51]
[4]  
CILLO F, 1995, 6 INT PLANT VIR EP S
[5]   ROLE OF SATELLITE RNA IN THE EXPRESSION OF SYMPTOMS CAUSED BY PLANT-VIRUSES [J].
COLLMER, CW ;
HOWELL, SH .
ANNUAL REVIEW OF PHYTOPATHOLOGY, 1992, 30 :419-442
[6]   NUCLEOTIDE-SEQUENCE OF A SATELLITE RNA OF A STRAIN OF CUCUMBER MOSAIC-VIRUS ASSOCIATED WITH A TOMATO FRUIT NECROSIS [J].
CRESCENZI, A ;
GRIECO, F ;
GALLITELLI, D .
NUCLEIC ACIDS RESEARCH, 1992, 20 (11) :2886-2886
[7]   CUCUMBER MOSAIC CUCUMOVIRUS POPULATIONS IN ITALY UNDER NATURAL EPIDEMIC CONDITIONS AND AFTER A SATELLITE-MEDIATED PROTECTION TEST [J].
CRESCENZI, A ;
BARBAROSSA, L ;
GALLITELLI, D ;
MARTELLI, GP .
PLANT DISEASE, 1993, 77 (01) :28-33
[8]   ROLE OF CUCUMBER MOSAIC-VIRUS AND ITS SATELLITE RNA IN THE ETIOLOGY OF TOMATO FRUIT NECROSIS IN ITALY [J].
CRESCENZI, A ;
BARBAROSSA, L ;
CILLO, F ;
DIFRANCO, A ;
VOVLAS, N ;
GALLITELLI, D .
ARCHIVES OF VIROLOGY, 1993, 131 (3-4) :321-333
[9]   CUCUMBER MOSAIC-VIRUS SATELLITE RNA (STRAIN-Y) - ANALYSIS OF SEQUENCES WHICH AFFECT SYSTEMIC NECROSIS ON TOMATO [J].
DEVIC, M ;
JAEGLE, M ;
BAULCOMBE, D .
JOURNAL OF GENERAL VIROLOGY, 1990, 71 :1443-1449
[10]  
Felsenstein J, 1989, Cladistics, V5, P164