Proteomic analysis of mature melanosomes from the retinal pigmented epithelium

被引：28

作者：

Azarian, SM

McLeod, I

Lillo, C

Gibbs, D

Yates, JR

Williams, DS

机构：

[1] Univ Calif San Diego, Sch Med, Dept Pharmacol, La Jolla, CA 92093 USA

[2] Univ Calif San Diego, Sch Med, Dept Neurosci, La Jolla, CA 92093 USA

[3] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 03期

关键词：

retinal pigment epithelium; melanosome; proteomics;

D O I：

10.1021/pr0502323

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The protein content of melanosomes in the retinal pigment epithelium (RPE) was analyzed by mass spectrometry. More than 100 proteins were found to be common to two out of three variations of sample preparation. Some proteins normally associated with other organelles were detected. Several lysosornal enzymes were detected, with the presence of cathepsin D confirmed by immunoelectron microscopy, thus supporting the previously suggested notion that melanosomes may contribute to the degradation of ingested photoreceptor outer segment disks.

引用

页码：521 / 529

页数：9

共 49 条

[1] Proteomic analysis of early melanosomes: Identification of novel melanosomal proteins [J].