Pharmacological characterization and neutralization of the venoms used in the production of Bothropic antivenom in Brazil

被引:62
作者
Camey, KU
Velarde, DT
Sanchez, EF [1 ]
机构
[1] Fundacao Ezequiel Dias, Ctr Pesquisa & Desenvolvimento, BR-30510010 Belo Horizonte, MG, Brazil
[2] Fundacao Ezequiel Dias, Div Imunobiol, BR-30510010 Belo Horizonte, MG, Brazil
关键词
snake venoms; antivenoms; Bothrops species; Brazil;
D O I
10.1016/S0041-0101(01)00245-8
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Some pharmacological effects of the venoms of five different Brazilian Bothrops species and a pool of these venoms (AgB) were quantified. The ability of polyspecific Bothropic antivenom produced at Fundacao Ezequiel Dias (FUNED, Brazil) to neutralize the principal toxic and enzymatic activities was studied using in vivo and in vitro assays. The lethality. hemorrhagic. necrotizing, proteolytic, phospholipase, coagulant and fibrinolytic activities were measured for each of these venoms. Comparison of protein electrophoretic patterns showed significant differences such as the presence of common and also unique components. Furthermore, experimental studies revealed differences in their biological properties among individual samples. It was found that the Bothrops antivenom was highly effective in the neutralization of the toxic activities of all venoms tested. In addition, indirect ELISA was used to compare the antigenic cross-reactivity for each of the five Bothrops venoms as well as the venoms of B. atrox. B. leucurus and B. erythromelas which were not included in the antigenic pool (AgB), Therefore. the characterization of several toxic activities of snake venoms is necessary, if toxicity is to be properly evaluated. Results indicate that the Brazilian antivenom prepared at FUNED against Bothrops snakes is effective in neutralizing the main toxic effects of Bothrops venoms. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:501 / 509
页数:9
相关论文
共 36 条
[1]  
[Anonymous], 2006, HERPETOZOA
[2]   BOTHROPS-ASPER VENOM FROM THE ATLANTIC AND PACIFIC ZONES OF COSTA-RICA [J].
ARAGON, F ;
GUBENSEK, F .
TOXICON, 1981, 19 (06) :797-805
[3]   HEMORRHAGIC METALLOPROTEINASES FROM SNAKE-VENOMS [J].
BJARNASON, JB ;
FOX, JW .
PHARMACOLOGY & THERAPEUTICS, 1994, 62 (03) :325-372
[4]  
CARDOSO JLC, 1993, Q J MED, V86, P315
[5]   SNAKE-VENOM VARIABILITY - METHODS OF STUDY, RESULTS AND INTERPRETATION [J].
CHIPPAUX, JP ;
WILLIAMS, V ;
WHITE, J .
TOXICON, 1991, 29 (11) :1279-1303
[6]  
Dias da Silva W., 1989, Memorias do Instituto Butantan, V51, P153
[7]   Action of metalloproteinases mutalysin I and II on several-components of the hemostatic and fibrinolytic systems [J].
Estêvao-Costa, MI ;
Diniz, CR ;
Magalhaes, A ;
Markland, FS ;
Sanchez, EF .
THROMBOSIS RESEARCH, 2000, 99 (04) :363-376
[8]  
Finney D.J., 1977, PROBIT ANAL, VIII
[9]   Bothrops sp. snake venoms:: Comparison of some biochemical and physicochemical properties and interference in platelet functions [J].
Francischetti, IMB ;
Castro, HC ;
Zingali, RB ;
Carlini, CR ;
Guimaraes, JA .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 1998, 119 (01) :21-29
[10]  
FREITAS TV, 1991, BRAZ J MED BIOL RES, V24, P281