Isolation and characterization of temperature-sensitive mutants of the Staphylococcus aureus dnaC gene

被引：14

作者：

Kaito, C ^{[1
]}

Kurokawa, K ^{[1
]}

Hossain, MS ^{[1
]}

Akimitsu, N ^{[1
]}

Sekimizu, K ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Pharmaceut Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 210卷 / 01期

关键词：

dnaC helicase; temperature-sensitive mutant; DNA replication; DNA repair; Staphylococcus aureus;

D O I：

10.1016/S0378-1097(02)00603-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A protein encoded by the Staphylococcus aureus dnaC gene has 44% and 58% homology with Escherichia coli DnaB and Bacillus subtilis DnaC replicative DNA helicases, respectively. We identified five mutant strains whose temperature-sensitive colony formation phenotypes were complemented by the dnaC gene. DNA replication in these mutants has a fast-stop phenotype, indicating that the S. aureus dnaC gene encodes the replicative DNA helicase required for the elongation step. These mutants were also sensitive to UV irradiation, suggesting that the dnaC gene is involved in DNA repair. The number of viable mutant cells decreased at a non-permissive temperature, suggesting that S. aureus DnaC helicase is a promising target for antibiotics providing bactericidal effects. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：157 / 164

页数：8

共 21 条

[1] TEMPERATURE-SENSITIVE DEOXYRIBONUCLEIC-ACID REPLICATION IN A DNA-C MUTANT OF BACILLUS-SUBTILIS [J].