Fluctuating estrogen and progesterone receptor expression in brainstem norepinephrine neurons through the rat estrous cycle

被引:131
作者
Haywood, SA
Simonian, SX
van der Beek, EM
Bicknell, RJ
Herbison, AE [1 ]
机构
[1] Babraham Inst, Neuroendocrinol Lab, Cambridge CB2 4AT, England
[2] Agr Univ Wageningen, EMV, Human & Anim Physiol Grp, Dept Anim Sci, NL-6709 Wageningen, Netherlands
关键词
D O I
10.1210/en.140.7.3255
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Norepinephrine (NE) neurons within the nucleus tractus solitarii (NTS; A2 neurons) and ventrolateral medulla (A1 neurons) represent gonadal steroid-dependent components of several neural networks regulating reproduction. Previous studies have shown that both A1 and A2 neurons express estrogen receptors (ERs). Using double labeling immunocytochemistry we report here that substantial numbers of NE neurons located within the NTS express progesterone receptor (PR) immunoreactivity, whereas few PRs are found in ventrolateral medulla. The evaluation of ER alpha and PR immunoreactivity in NE neurons through the estrous cycle revealed a fluctuating pattern of expression for both receptors within the NTS. The percentage of A2 neurons expressing PR immunoreactivity was low on metestrus and diestrus (3-7%), but increased significantly to approximately 24% an proestrous morning and remained at intermediate levels until estrus. The pattern of ER alpha immunoreactivity in A2 neurons was more variable, but a similar increment from 11% to 40% of NE neurons expressing ER alpha was found from diestrus to proestrus. Experiments in ovariectomized, estrogen-treated and estrogen-plus progesterone-treated rats revealed that PR immunoreactivity in A2 neurons was induced strongly by estrogen treatment, whereas progesterone had no significant effect. The numbers of ER alpha-positive NE neurons were not influenced by steroid treatment. These observations provide direct evidence for PRs in NE neurons of the brainstem and show that cyclical patterns of gonadal steroid receptor expression exist in A2, but not A1, neurons through the rat estrous cycle. The expression of PR in A2 neurons appears to be driven principally by circulating estrogen concentrations. The fluctuating levels of ER alpha and PR expression in these brainstem NE neurons may help generate cyclical patterns of biosynthetic and electrical activity within reproductive neural networks.
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页码:3255 / 3263
页数:9
相关论文
共 47 条
[1]  
BARRACLOUGH CA, 1982, ENDOCR REV, V3, P91, DOI 10.1210/edrv-3-1-91
[2]   ELECTRICAL-STIMULATION OF ASCENDING NORADRENERGIC TRACTS IN THE MIDBRAIN - SUPPRESSION OF STEROID-INDUCED LUTEINIZING-HORMONE RELEASE [J].
BERGEN, H ;
LEUNG, PCK .
ENDOCRINOLOGY, 1988, 122 (03) :899-905
[3]   ESTRADIOL-INDUCED PROGESTIN RECEPTOR IMMUNOREACTIVITY IS FOUND ONLY IN ESTROGEN RECEPTOR-IMMUNOREACTIVE CELLS IN GUINEA-PIG BRAIN [J].
BLAUSTEIN, JD ;
TURCOTTE, JC .
NEUROENDOCRINOLOGY, 1989, 49 (05) :454-461
[4]   STUDIES ON THE NEURONAL SYSTEMS INVOLVED IN THE ESTROGEN-NEGATIVE FEEDBACK EFFECT ON GONADOTROPIN-RELEASING-HORMONE NEURONS IN THE EWE [J].
CLARKE, IJ ;
SCOTT, CJ .
HUMAN REPRODUCTION, 1993, 8 :2-6
[5]   CHANGING PATTERNS OF FOS EXPRESSION IN BRAIN-STEM CATECHOLAMINERGIC NEURONS DURING THE RAT ESTROUS-CYCLE [J].
CONDE, GL ;
BICKNELL, RJ ;
HERBISON, AE .
BRAIN RESEARCH, 1995, 672 (1-2) :68-76
[6]  
CURRAN MA, 1998, SOC NEUR ABSTR 7362, V24
[7]   NORADRENERGIC AND DOPAMINERGIC PROJECTIONS TO THE MEDIAL PREOPTIC AREA OF THE RAT - A COMBINED HORSERADISH PEROXIDASE-CATECHOLAMINE FLUORESCENCE STUDY [J].
DAY, TA ;
BLESSING, W ;
WILLOUGHBY, JO .
BRAIN RESEARCH, 1980, 193 (02) :543-548
[8]   PREOPTIC CATECHOLAMINE, GABA, AND GLUTAMATE RELEASE IN OVARIECTOMIZED AND OVARIECTOMIZED ESTROGEN-PRIMED RATS UTILIZING A PUSH-PULL CANNULA TECHNIQUE [J].
DEMLING, J ;
FUCHS, E ;
BAUMERT, M ;
WUTTKE, W .
NEUROENDOCRINOLOGY, 1985, 41 (03) :212-218
[9]   ESTRADIOL AND PROGESTERONE MODULATION OF NOREPINEPHRINE NEUROTRANSMISSION - IMPLICATIONS FOR THE REGULATION OF FEMALE REPRODUCTIVE-BEHAVIOR [J].
ETGEN, AM ;
UNGAR, S ;
PETITTI, N .
JOURNAL OF NEUROENDOCRINOLOGY, 1992, 4 (03) :255-271
[10]   ANALYSIS OF BRAIN-STEM A1 AND A2 NORADRENERGIC INPUTS TO THE PREOPTIC AREA USING MICRODIALYSIS IN THE RAT [J].
FERNANDEZGALAZ, C ;
DYER, RG ;
HERBISON, AE .
BRAIN RESEARCH, 1994, 636 (02) :227-232