Direct measurement of interactions between tethered poly(ethylene glycol) chains and adsorbed mucin layers

被引:85
作者
Efremova, NV
Huang, Y
Peppas, NA [1 ]
Leckband, DE
机构
[1] Univ Illinois, Dept Chem Engn, Urbana, IL 61801 USA
[2] Purdue Univ, Sch Chem Engn, W Lafayette, IN 47907 USA
[3] Purdue Univ, Dept Biomed Engn, W Lafayette, IN 47907 USA
关键词
D O I
10.1021/la011303p
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Direct force measurements were used to investigate the interactions between grafted poly(ethylene glycol) (PEG) (M-W 2000) and both adsorbed and soluble bovine submaxillary gland mucin. These measurements show that both soluble and adsorbed mucin adhere weakly to the grafted PEG layers. Soluble mucin mediates a concentration-dependent, bridging attraction between the PEG films. Similarly, PEG adheres to adsorbed mucin layers in both the presence and absence of 0.2 mg/mL mucin solutions. The heteropolymer attraction is pH dependent, with the adhesion being substantially higher at pH 2 than at pH 7.2. The latter suggests that hydrogen-bonding interactions are responsible for binding. The weaker mucin interactions at pH 7.2, however, allow for PEG-coated surfaces to adhere to surface-bound mucin in the presence of soluble mucin. Such characteristics are particularly desirable for coatings for oral drug carriers.
引用
收藏
页码:836 / 845
页数:10
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