An electrochemical immunosensor using ferrocenyl-tethered dendrimer

被引:47
作者
Kwon, SJ
Kim, E
Yang, H
Kwak, J [1 ]
机构
[1] Pusan Natl Univ, Dept Chem, Pusan, South Korea
[2] Korea Adv Inst Sci & Technol, Dept Chem, Taejon 305701, South Korea
[3] Pusan Natl Univ, Ctr Innovat Biophysio Sensor Technol, Pusan, South Korea
关键词
D O I
10.1039/b509969b
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
We report here an enzyme-amplified, sandwich-type immunosensor for detecting the biospecific interaction between an antibody and antigen using redox mediation. We employed biotin/anti-biotin IgG as a model immunosensing pair. Partially ferrocenyl-tethered dendrimer (Fc-D), whose ferrocene moiety acts as a redox mediator, was immobilized to the electrode surface by covalent binding between the dendrimer amines and the carboxylic acids of a self-assembled monolayer. The unreacted amines of the immobilized Fc-D were modified with biotin groups to allow the specific binding of goat anti-biotin IgG. Rabbit anti-goat IgG-conjugated alkaline phosphatase was bound to goat anti-biotin IgG to catalyze conversion of p-aminophenyl phosphate monohydrate to p-aminophenol. This product is oxidized to quinoimide by the reduction of ferrocenium back to ferrocene, producing an electrocatalytic anodic current. Cyclic voltammograms and surface plasmon resonance experiments showed that the binding of nonspecific proteins is not significant on the biotinylated Fc-D surface. We also examined the change in peak current according to the concentration of anti- biotin IgG and found that the detection range of this immunosensing scheme is between 0.1 and 30 mu g mL(-1).
引用
收藏
页码:402 / 406
页数:5
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