Generating regionalized neuronal cells from pluripotency, a step-by-step protocol

被引：38

作者：

Kirkeby, Agnete ^{[1
,2
]}

Nelander, Jenny

Parmar, Malin

机构：

[1] Lund Univ, Dept Expt Med Sci, SE-22184 Lund, Sweden

[2] Lund Univ, Lund Stem Cell Ctr, SE-22184 Lund, Sweden

来源：

FRONTIERS IN CELLULAR NEUROSCIENCE | 2013年 / 6卷

基金：

瑞典研究理事会;

关键词：

human embryonic stem cells; pluripotent stem cells; differentiation; protocol; neuronal subtypes; regionalization; GSK3; brain development; DOPAMINE NEURONS; HUMAN ES; DERIVATION;

D O I：

10.3389/fncel.2012.00064

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.

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