Ischemic brain cell-derived conditioned medium protects astrocytes against ischemia through GDNF/ERK/NF-kB signaling pathway

被引：38

作者：

Chu, Lan-Feng ^{[1
]}

Wang, Wei-Ti ^{[1
]}

Ghanta, Vithal K. ^{[2
]}

Lin, Chi-Hsin ^{[1
,3
]}

Chiang, Yung-Yen ^{[4
]}

Hsueh, Chi-Mei ^{[1
]}

机构：

[1] Natl Chung Hsing Univ, Dept Life Sci, Taichung 40227, Taiwan

[2] Univ Alabama Birmingham, Dept Biol, Birmingham, AL 35294 USA

[3] Taipei Med Univ, Grad Inst Pharmacol, Taipei, Taiwan

[4] Cent Taiwan Univ Sci & Technol, Dept Dent Lab Technol, Taichung, Taiwan

来源：

BRAIN RESEARCH | 2008年 / 1239卷

关键词：

In vitro ischemia; Astrocyte; GDNF; ERK; NF-kB;

D O I：

10.1016/j.brainres.2008.08.087

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Conditioned medium (CM) collected from cultures of ischemic microglia, astrocytes, and neurons were protective to astrocytes under the in vitro ischemic condition (deprivation of oxygen, glucose and serum). Molecular and signaling pathway(s) responsible for the CMs protective activity were investigated. Results showed that CMs from the ischemic microglia (MCM), astrocytes (ACM) and neurons (NCM) contained glial cell line-derived neurotrophic factor (GDNF), which protects astrocytes against the in vitro ischernia. Expression of extra cellular signal-regulated kinase (ERK1/2) and nuclear factor-kappa B (NF-kB) by GDNF led to the inhibition of apoptosis of the ischemic astrocytes in a caspase 3-independent manner. However, CMs- and GDNF-mediated protection of the ischemic astrocytes was protein kinase B (Akt) independent. These results provided mechanistic data regarding how GDNF and CMs-mediated protection of the ischemic astrocytes is taking place. These observations provide information for the use of GDNF and GDNF containing CMs in the control of cerebral ischemia. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：24 / 35

页数：12

共 36 条

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