Metabolomics with LC-QTOF-MS Permits the Prediction of Disease Stage in Aortic Abdominal Aneurysm Based on Plasma Metabolic Fingerprint

被引:60
作者
Ciborowski, Michal [1 ,2 ]
Teul, Joanna [1 ,3 ]
Luis Martin-Ventura, Jose [4 ,5 ]
Egido, Jesus [4 ,5 ]
Barbas, Coral [1 ]
机构
[1] Univ CEU San Pablo, Fac Farm, CEMBIO Ctr Metabol & Bioanal, Madrid, Spain
[2] Med Univ Bialystok, Dept Phys Chem, Bialystok, Poland
[3] Med Univ Bialystok, Dept Pharmaceut Anal, Bialystok, Poland
[4] IIS Fdn Jimenez Diaz, Vasc Res Lab, Madrid, Spain
[5] Univ Autonoma Madrid, Madrid, Spain
来源
PLOS ONE | 2012年 / 7卷 / 02期
关键词
PATHOGENESIS; PERFORMANCE; CARNITINE;
D O I
10.1371/journal.pone.0031982
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Abdominal aortic aneurysm (AAA) is a permanent and localized aortic dilation, defined as aortic diameter >= 3 cm. It is an asymptomatic but potentially fatal condition because progressive enlargement of the abdominal aorta is spontaneously evolving towards rupture. Biomarkers may help to explain pathological processes of AAA expansion, and allow us to find novel therapeutic strategies or to determine the efficiency of current therapies. Metabolomics seems to be a good approach to find biomarkers of AAA. In this study, plasma samples of patients with large AAA, small AAA, and controls were fingerprinted with LC-QTOF-MS. Statistical analysis was used to compare metabolic fingerprints and select metabolites that showed a significant change. Results presented here reveal that LC-QTOF-MS based fingerprinting of plasma from AAA patients is a very good technique to distinguish small AAA, large AAA, and controls. With the use of validated PLS-DA models it was possible to classify patients according to the disease stage and predict properly the stage of additional AAA patients. Identified metabolites indicate a role for sphingolipids, lysophospholipids, cholesterol metabolites, and acylcarnitines in the development and progression of AAA. Moreover, guanidinosuccinic acid, which mimics nitric oxide in terms of its vasodilatory action, was found as a strong marker of large AAA.
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页数:9
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