RNA interference targeting the platelet-derived growth factor receptor β subunit ameliorates experimental hepatic fibrosis in rats

被引：52

作者：

Chen, Si-Wen ^{[1
]}

Zhang, Xing-Rong ^{[1
]}

Wang, Chong-Ze ^{[2
]}

Chen, Wei-Zhong ^{[1
]}

Xie, Wei-Fen ^{[1
]}

Chen, Yue-Xiang ^{[1
]}

机构：

[1] Second Mil Med Univ, Changzheng Hosp, Dept Gastroenterol, Shanghai 200003, Peoples R China

[2] Shanghai Jiao Tong Univ, Renji Hosp, Dept Geriatr, Shanghai 200030, Peoples R China

来源：

LIVER INTERNATIONAL | 2008年 / 28卷 / 10期

基金：

中国国家自然科学基金;

关键词：

cell proliferation; gene therapy; hepatic fibrosis; hepatic stellate cells; platelet-derived growth factor receptor beta subunit (PDGFR-beta); RNA interference;

D O I：

10.1111/j.1478-3231.2008.01759.x

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background/Aims: Platelet-derived growth factor (PDGF) is the strongest stimulator of the proliferation of hepatic stellate cells (HSCs). PDGF receptor beta subunit (PDGFR-beta) is acquired on HSCs proliferation induced by PDGF. In this study, we aim to investigate the effect of PDGFR-beta small interference RNA (siRNA) on experimental hepatic fibrosis. Methods: We constructed a PDGFR-beta siRNA expression plasmid and investigated its effect on the activation of HSCs. Bromodeoxyuridine incorporation was performed to investigate the effect of PDGFR-beta siRNA on HSCs proliferation. A hydrodynamics-based transfection method was used to deliver PDGFR-beta siRNA to rats with hepatic fibrosis. The distribution of transgenes in the liver was observed by immunofluorescence. The antifibrogenic effect of PDGFR-beta siRNA was investigated pathologically. Results: Platelet-derived growth factor receptor-beta subunit siRNA could significantly downregulate PDGFR-beta expression, suppress HSCs activation, block the mitogen-activated protein kinase signalling pathway and inhibit HSCs proliferation in vitro. PDGFR-beta siRNA expression plasmid could be delivered into activated HSCs by the hydrodynamics-based transfection method, and remarkably improve the liver function of the rat model induced by dimethylnitrosamine and bile duct ligation. Furthermore, the progression of fibrosis in the liver was significantly suppressed by PDGFR-beta siRNA in both animal models. Conclusions: Platelet-derived growth factor receptor-beta subunit siRNA may be presented as an effective antifibrogenic gene therapeutic method for hepatic fibrosis.

引用

页码：1446 / 1457

页数：12

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