Structure of cell clusters formed in cultures of dissociated human embryonic brain

被引：13

作者：

Revishchin, AV

Poltavtseva, RA

Marei, MV

Aleksandrova, MA

Viktorov, IV

Korochkin, LI

Sukhikh, GT

机构：

[1] Russian Acad Sci, Inst Gene Biol, Moscow, Russia

[2] Russian Acad Sci, AN Severtsov Inst Ecol & Evolut Problems, Moscow, Russia

[3] Russian Acad Sci, NK Koltsov Dev Biol Inst, Moscow, Russia

[4] Russian Acad Med Sci, Inst Brain, Moscow 109801, Russia

[5] Inst Med Biol, Moscow, Russia

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2001年 / 132卷 / 03期

关键词：

stem cells; cell clusters; cultured embryonic cells; human;

D O I：

10.1023/A:1013118717866

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Cell clusters in a culture of dissociated brain from human fetuses at 8-12 weeks gestation in a serum-free growth medium were studied by immunohistochemical methods and electron microscopy. Heterogeneity of cell population in culture was demonstrated. Despite the influence of proliferation-stimulating factors, cell clusters contained not only nestin-immunopositive stem cells, but also beta-tubulin-, vimentin-, and GFAP-positive cells differentiating by the neural pathway. Stem cells were localized on the surface of clusters. The percentage of stem cells in large clusters was lower than in small clusters.

引用

页码：856 / 860

页数：5

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