TNF-α is involved in activating DNA fragmentation in skeletal muscle

被引：61

作者：

Carbó, N ^{[1
]}

Busquets, S ^{[1
]}

van Royen, M ^{[1
]}

Alvarez, B ^{[1
]}

López-Soriano, FJ ^{[1
]}

Argilés, JM ^{[1
]}

机构：

[1] Univ Barcelona, Fac Biol, Dept Bioquim & Biol Mol, Canc Res Grp, E-08028 Barcelona, Spain

来源：

BRITISH JOURNAL OF CANCER | 2002年 / 86卷 / 06期

关键词：

DNA fragmentation; skeletal muscle; TNF-alpha; cancer cachexia; apoptosis;

D O I：

10.1038/sj.bjc.6600167

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Intraperitoneal administration of 100 mug kg(-1) (body weight) of tumour necrosis factor-alpha to rats for 8 consecutive days resulted in a significant decrease in protein content, which was concomitant with a reduction in DNA content. Interestingly, the protein/DNA ratio was unchanged in the skeletal muscle of the tumour necrosis facto-alpha-treated animals as compared with the non-treated controls. Analysis of muscle DNA fragmentation clearly showed enhanced laddering in the skeletal muscle of tumour necrosis factor-alpha-treated animals, suggesting an apoptotic phenomenon, In a different set of experiments, mice bearing a cachexia-inducing tumour (the Lewis lung carcinoma) showed an increase in muscle DNA fragmentation (9.8-fold) as compared with their non-tumour-bearing control counterparts as previously described. When gene-deficient mice for tumour necrosis factor-alpha receptor protein I were inoculated with Lewis lung carcinoma, they were also affected by DNA fragmentation; however the increase was only 2.1-fold. These results suggest that tumour necrosis factor-alpha partly mediates DNA fragmentation during experimental cancer-associated cachexia. (C) 2002 Cancer Research UK.

引用

页码：1012 / 1016

页数：5

共 51 条

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