Inhibition of Prevotella and Capnocytophaga immunoglobulin A1 proteases by human serum

Oral Prevotella and Capnocytophaga species, regularly isolated from periodontal pockets and associated with extraoral infections, secrete specific immunoglobulin A1 (IgA1) proteases cleaving human IgA1 in the hinge region into intact Fab and Fc fragments, To investigate whether these enzymes are subject to inhibition in vivo in humans, we tested 34 sera from periodontally diseased and healthy individuals in an enzyme-linked immunosorbent assay for the presence and titers of inhibition of seven Prevotella and Capnocytophaga proteases. All or nearly all of the sera inhibited the IgA1 protease activity of Prevotella buccae, Prevotella or is, and Prevotella loescheii, A minor proportion of the sera inhibited Prevotella buccae, Prevotella denticola, and Prevotella melaninogenica IgA1 proteases, while no sera inhibited Capnocytophaga ochracea IgA1 protease. All inhibition titers were low, ranging from 5 to 55, with titer being defined as the reciprocal of the dilution of serum causing 50% inhibition of one defined unit of protease activity, No correlation between periodontal disease status and the presence, absence, or titer of inhibition cr as observed, The nature of the low titers of inhibition in all sera of the IgA1 proteases of P. buccae, P. oris, and P. loescheii was further examined, In size exclusion chromatography, inhibitory activity corresponded to the peak volume of IgA, Additional inhibition of the P. oris IgA1 protease was found in fractions containing both IgA and IgG, Purification of the IgG fractions of five sera by passage of the sera on a protein G column resulted in recovery of inhibitory IgG antibodies against all three IgA1 proteases, with the highest titer being for the P. oris enzyme, These findings indicate that inhibitory activity is associated with enzyme-neutralizing antibodies.