S-RNases in apple are expressed in the pistil along the pollen tube growth path

被引:36
作者
Certal, AC
Sanchez, AM
Kokko, H
Broothaerts, W
Oliveira, MM
Feijó, JA
机构
[1] Univ Nova Lisboa, Fac Ciencias, Dept Biol Vegetal, P-1749016 Lisbon, Portugal
[2] Univ Kuopio, Dept Biochem & Biotechnol, Bot Garden, SF-70211 Kuopio, Finland
[3] Katholieke Univ Leuven, Fruitteelctr, B-3001 Heverlee, Belgium
[4] Inst Biol Expt & Tecnol, Lab Engn Genet Plantas, P-2780 Oeiras, Portugal
来源
SEXUAL PLANT REPRODUCTION | 1999年 / 12卷 / 02期
关键词
confocal; gametophytic self-incompatibility; immuno-histochemistry; Malus domestica; S-RNases;
D O I
10.1007/s004970050177
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The molecular bases of self-incompatibility have been intensively studied in a restricted number of model species, but for most families the expression and distribution of S-proteins is unknown. In this work, pistil cryosections from apple were used for in situ detection of S-proteins. Two specific antibodies, one against the S3-protein and another against all apple S-proteins were used. S-proteins were shown to be localised in the intercellular space of the transmitting tissue, both in the stigma and style, which agrees with the proposed mechanism of action for S-RNases in gametophytic self-incompatibility. Some intracellular labelling was also observed in all ovary sections, confined to one layer of the nucellus surrounding the embryo sac, but this labelling was found to be non-S-allele-specific. Nevertheless, the signal in the ovary was tissue-specific, which may indicate that some component not encoded by the S-locus but similar to S-proteins was detected. To the best of our knowledge this is the first report on the precise distribution of S-RNases in a rosaceous species.
引用
收藏
页码:94 / 98
页数:5
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