Effects of endothelin-3 on intestinal ion transport

被引：7

作者：

Bosc, LVG ^{[1
]}

Majowicz, MP ^{[1
]}

Ortiz, MC ^{[1
]}

Vidal, NA ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Farm & Bioquim, Catedra Biol Celular & Histol, RA-1113 Buenos Aires, DF, Argentina

来源：

PEPTIDES | 2001年 / 22卷 / 12期

关键词：

endothelin; 3; small intestine; ion transport; nitric oxide; SGLT1;

D O I：

10.1016/S0196-9781(01)00554-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the effects of endothelin 3 (ET-3) on electrolyte transport in rat small intestine using a voltage clamp technique in Ussing's chamber. ET-3 diminished potential difference (PD) and short circuit current (Ise). ET-3 did not affect PD or Ise in low Na(+) and/or D-glucose-free medium. Phloridzine (an inhibitor of sodium-glucose cotransporter [SGLT1]) pretreatment abolished the effect of ET-3 on Ise. Methylene blue (a soluble guanylate cyclase inhibitor) or N-nitro-L-arginine methyl ester (a NOS inhibitor) pretreatment delayed the effect of ET-3 on PD and Isc. ET-3 enhanced NOS activity on enterocytes and systemic NO production. Then, ET-3 could inhibit SGLT1 with the participation of NO. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：2069 / 2075

页数：7

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