Simultaneous differentiation and typing of Entamoeba histolytica and Entamoeba dispar

被引：47

作者：

Zaki, M ^{[1
]}

Meelu, P ^{[1
]}

Sun, W ^{[1
]}

Clark, CG ^{[1
]}

机构：

[1] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Dis, London WC1E 7HT, England

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2002年 / 40卷 / 04期

关键词：

D O I：

10.1128/JCM.40.4.1271-1276.2002

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Sequences corresponding to some of the polymorphic loci previously reported from Entamoeba histolytica have been detected in Entamoeba dispar. Comparison of nucleotide sequences of two loci between E. dispar strain SAW760 and E. histolytica strain HM-1:IMSS revealed significant differences in both repeat and flanking regions. The tandem repeat units varied not only in sequence but also in number and arrangement between the two species at both the loci. Using the sequences obtained, primer pairs aimed at amplifying species-specific products were designed and tested on a variety of E. histolytica and E. dispar samples. Amplification results were in complete agreement with the original species classification in all cases, and the PCR products displayed discernible size and pattern variations among the isolates.

引用

页码：1271 / 1276

页数：6

共 23 条

[1] POLYMERASE CHAIN REACTION-SOLUTION HYBRIDIZATION ENZYME-LINKED IMMUNOASSAY (PCR-SHELA) FOR THE DIFFERENTIAL-DIAGNOSIS OF PATHOGENIC AND NONPATHOGENIC ENTAMOEBA-HISTOLYTICA [J].

AGUIRRE, A ;

WARHURST, DC ;

GUHL, F ;

FRAMER, IA .

TRANSACTIONS OF THE ROYAL SOCIETY OF TROPICAL MEDICINE AND HYGIENE, 1995, 89 (02) :187-188

[2]

[Anonymous], 1997, B WHO, V75, P291

[3] ENTAMOEBA-HISTOLYTICA - ELECTROPHORETIC ISOENZYME PATTERNS OF STRAINS AND THEIR VIRULENCE IN THE CECUM OF GERBILS (MERIONES-UNGUICULATUS) [J].

CHADEE, K ;

SMITH, JM ;

MEEROVITCH, E .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1985, 34 (05) :870-878

[4]

CLARK CG, 1991, MOL BIOCHEM PARASIT, V46, P11, DOI 10.1016/0166-6851(91)90194-B

[5] RIBOSOMAL-RNA GENES OF PATHOGENIC AND NONPATHOGENIC ENTAMOEBA-HISTOLYTICA ARE DISTINCT [J].

CLARK, CG ;

DIAMOND, LS .

MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 1991, 49 (02) :297-302

[6]

CLARK CG, IN PRESS CLIN MICROB

[7]

CLARKE JB, 1992, CLIN ANESTHESIA UPDA, V3, P1

[8] A REDESCRIPTION OF ENTAMOEBA-HISTOLYTICA SCHAUDINN, 1903 (EMENDED WALKER, 1911) SEPARATING IT FROM ENTAMOEBA-DISPAR BRUMPT, 1925 [J].

DIAMOND, LS ;

CLARK, CG .

JOURNAL OF EUKARYOTIC MICROBIOLOGY, 1993, 40 (03) :340-344

[9]

Espinosa Cantellano M., 1997, Archives of Medical Research, V28, pS204

[10] Entamoeba dispar:: Ultrastructure, surface properties and cytopathic effect [J].

Espinosa-Cantellano, M ;

González-Robles, A ;

Chávez, B ;

Castañón, G ;

Argüello, C ;

Lázaro-Haller, A ;

Martínez-Palomo, A .

JOURNAL OF EUKARYOTIC MICROBIOLOGY, 1998, 45 (03) :265-272

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