Directed enzyme evolution

被引：210

作者：

Farinas, ET ^{[1
]}

Bulter, T ^{[1
]}

Arnold, FH ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn 210 41, Pasadena, CA 91125 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 2001年 / 12卷 / 06期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S0958-1669(01)00261-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Laboratory evolutionists continue to generate better enzymes for industrial and research applications. Exciting developments include new biocatalysts for enantioselective carbon-carbon bond formation and fatty acid production in plants. Creative contributions to the repertoire of evolutionary methods will ensure further growth in applications and expand the scope and complexity of biological design problems that Gan be addressed. Researchers are also starting to elucidate mechanisms of enzyme adaptation and natural evolution by testing evolutionary scenarios in the laboratory.

引用

页码：545 / 551

页数：7

共 59 条

[1] High efficiency family shuffling based on multi-step PCR and in vivo DNA recombination in yeast: statistical and functional analysis of a combinatorial library between human cytochrome P450 1A1 and 1A2 [J].

Abecassis, Valerie ;

Pompon, Denis ;

Truan, Gilles .

NUCLEIC ACIDS RESEARCH, 2000, 28 (20) :E88

[2]

AMTSUMURA I, 2001, J MOL BIOL, V305, P331

[3] Combinatorial and computational challenges for biocatalyst design [J].

Arnold, FH .

NATURE, 2001, 409 (6817) :253-257

[4] How enzymes adapt: lessons from directed evolution [J].

Arnold, FH ;

Wintrode, PL ;

Miyazaki, K ;

Gershenson, A .

TRENDS IN BIOCHEMICAL SCIENCES, 2001, 26 (02) :100-106

[5]

ARNOLD FH, 2001, NATURE, V55

[6] Substrate-dependent mutant complementation to select fatty acid desaturase variants for metabolic engineering of plant seed oils [J].

Cahoon, EB ;

Shanklin, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (22) :12350-12355

[7] DNA shuffling method for generating highly recombined genes and evolved enzymes [J].

Coco, WM ;

Levinson, WE ;

Crist, MJ ;

Hektor, HJ ;

Darzins, A ;

Pienkos, PT ;

Squires, CH ;

Monticello, DJ .

NATURE BIOTECHNOLOGY, 2001, 19 (04) :354-359

[8] Application of a very high-throughput digital imaging screen to evolve the enzyme galactose oxidase [J].

Delagrave, S ;

Murphy, DJ ;

Pruss, JLR ;

Maffia, AM ;

Marrs, BL ;

Bylina, EJ ;

Coleman, WJ ;

Grek, CL ;

Dilworth, MR ;

Yang, MM ;

Youvan, DC .

PROTEIN ENGINEERING, 2001, 14 (04) :261-267

[9]

Faber K., 2000, Biotransformations in Organic Chemistry, V4th

[10] Efficient gene targeted random mutagenesis in genetically stable Escherichia coli strains [J].

Fabret, Celine ;

Poncet, Sandrine ;

Danielsen, Steffen ;

Borchert, Torben V. ;

Ehrlich, S. Dusko ;

Janniere, Laurent .

NUCLEIC ACIDS RESEARCH, 2000, 28 (21) :95

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