Structural analysis of the lipopolysaccharide from the nontypable Haemophilus influenzae strain SB 33

被引：30

作者：

Cox, AD ^{[1
]}

Masoud, H ^{[1
]}

Thibault, P ^{[1
]}

Brisson, JR ^{[1
]}

van der Zwan, M ^{[1
]}

Perry, MB ^{[1
]}

Richards, JC ^{[1
]}

机构：

[1] Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 2001年 / 268卷 / 20期

关键词：

nontypable Haemophilus influenzae; LPS; phase variation; NMR; mass spectrometry;

D O I：

10.1046/j.0014-2956.2001.02459.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of the core region of the lipopolysaccharide (LPS) from the nontypable Haemophilus influenzae strain SB 33 was elucidated. The LPS was subjected to a variety of degradative procedures. The structures of the derived oligosaccharide products were established by monosaccharide and methylation analyses, NMR spectroscopy and mass spectrometry. These analyses revealed a series of related phosphocholine (PCho) containing structures differing in the number of hexose residues. The results pointed to each species containing a conserved phosphoethanolamine (P Etn) substituted heptose-containing trisaccharide inner-core moiety. The major LPS glycoforms were identified as 2-Hex, 3-Hex and 4-Hex species according to the number of hexose residues present.

引用

页码：5278 / 5286

页数：9

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