Tricellulin constitutes a novel barrier at tricellular contacts of epithelial cells

被引:610
作者
Ikenouchi, J
Furuse, M
Furuse, K
Sasaki, H
Tsukita, S [1 ]
Tsukita, S [1 ]
机构
[1] Kyoto Univ, Fac Med, Dept Cell Biol, Kyoto 6068501, Japan
[2] Kyoto Univ, Fac Med, Sch Hlth Sci, Kyoto 6068501, Japan
[3] Japan Sci & Technol Corp, Solut Oriented Res Sci & Technol, Kyoto 6068501, Japan
[4] Knowledge Action Network Res Inst Inc, Kyoto 6008317, Japan
[5] Jikei Univ, Sch Med, Dept Mol Cell Biol, Inst DNA Med, Tokyo 105, Japan
关键词
D O I
10.1083/jcb.200510043
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
For epithelia to function as barriers, the intercellular space must be sealed. Sealing two adjacent cells at bicellular tight junctions (bTJs) is well described with the discovery of the claudins. Yet, there are still barrier weak points at tricellular contacts, where three cells join together. In this study, we identify tricellulin, the first integral membrane protein that is concentrated at the vertically oriented TJ strands of tricellular contacts. When tricellulin expression was suppressed with RNA interference, the epithelial barrier was compromised, and tricellular contacts and bTJs were disorganized. These findings indicate the critical function of tricellulin for formation of the epithelial barrier.
引用
收藏
页码:939 / 945
页数:7
相关论文
共 25 条
[1]   Setting up a selective barrier at the apical junction complex [J].
Anderson, JM ;
Van Itallie, CM ;
Fanning, AS .
CURRENT OPINION IN CELL BIOLOGY, 2004, 16 (02) :140-145
[2]   Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues [J].
AndoAkatsuka, Y ;
Saitou, M ;
Hirase, T ;
Kishi, M ;
Sakakibara, A ;
Itoh, M ;
Yonemura, S ;
Furuse, M ;
Tsukita, S .
JOURNAL OF CELL BIOLOGY, 1996, 133 (01) :43-47
[3]   A system for stable expression of short interfering RNAs in mammalian cells [J].
Brummelkamp, TR ;
Bernards, R ;
Agami, R .
SCIENCE, 2002, 296 (5567) :550-553
[4]   JUNCTIONAL COMPLEXES IN VARIOUS EPITHELIA [J].
FARQUHAR, MG ;
PALADE, GE .
JOURNAL OF CELL BIOLOGY, 1963, 17 (02) :375-&
[5]   VARIATIONS IN TIGHT AND GAP JUNCTIONS IN MAMMALIAN TISSUES [J].
FRIEND, DS ;
GILULA, NB .
JOURNAL OF CELL BIOLOGY, 1972, 53 (03) :758-+
[6]  
FUJIMOTO K, 1995, J CELL SCI, V108, P3443
[7]   A single gene product, claudin-1 or -2, reconstitutes tight junction strands and recruits occludin in fibroblasts [J].
Furuse, M ;
Sasaki, H ;
Fujimoto, K ;
Tsukita, S .
JOURNAL OF CELL BIOLOGY, 1998, 143 (02) :391-401
[8]   OCCLUDIN - A NOVEL INTEGRAL MEMBRANE-PROTEIN LOCALIZING AT TIGHT JUNCTIONS [J].
FURUSE, M ;
HIRASE, T ;
ITOH, M ;
NAGAFUCHI, A ;
YONEMURA, S ;
TSUKITA, S ;
TSUKITA, S .
JOURNAL OF CELL BIOLOGY, 1993, 123 (06) :1777-1788
[9]   Claudin-1 and -2: Novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin [J].
Furuse, M ;
Fujita, K ;
Hiiragi, T ;
Fujimoto, K ;
Tsukita, S .
JOURNAL OF CELL BIOLOGY, 1998, 141 (07) :1539-1550
[10]   DIRECT ASSOCIATION OF OCCLUDIN WITH ZO-1 AND ITS POSSIBLE INVOLVEMENT IN THE LOCALIZATION OF OCCLUDIN AT TIGHT JUNCTIONS [J].
FURUSE, M ;
ITOH, M ;
HIRASE, T ;
NAGAFUCHI, A ;
YONEMURA, S ;
TSUKITA, S ;
TSUKITA, S .
JOURNAL OF CELL BIOLOGY, 1994, 127 (06) :1617-1626