Engineering Bacillus subtilis ATCC 6633 for improved production of the lantibiotic subtilin

被引：55

作者：

Heinzmann, S ^{[1
]}

Entian, KD ^{[1
]}

Stein, T ^{[1
]}

机构：

[1] Goethe Univ Frankfurt, Inst Mikrobiol, D-60439 Frankfurt, Germany

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2006年 / 69卷 / 05期

关键词：

D O I：

10.1007/s00253-005-0023-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

To improve the production of the lantibiotic subtilin in Bacillus subtilis ATCC 6633, two genetic engineering strategies were followed. Firstly, additional copies of subtilin self-protection (immunity) genes spaIFEG have been integrated into the genome of the producer strain. Their expression significantly enhanced the subtilin tolerance level, and concomitantly, the subtilin yield 1.7-fold. Secondly, a repressor of subtilin gene expression, the B. subtilis general transition state regulator protein AbrB, was deleted. A sixfold enhancement of the subtilin yield could be achieved with the abrB deletion mutant; however, the produced subtilin fraction predominantly consists of succinylated subtilin species with less antimicrobial activity compared to unmodified subtilin.

引用

页码：532 / 536

页数：5

共 30 条

[1] REQUIREMENTS FOR TRANSFORMATION IN BACILLUS SUBTILIS [J].

ANAGNOSTOPOULOS, C ;

SPIZIZEN, J .

JOURNAL OF BACTERIOLOGY, 1961, 81 (05) :741-&

[2]

BIRNBOIM HC, 1979, NUCLEIC ACIDS RES, V7, P1513

[3] Use of the cell wall precursor lipid II by a pore-forming peptide antibiotic [J].

Breukink, E ;

Wiedemann, I ;

van Kraaij, C ;

Kuipers, OP ;

Sahl, HG ;

de Kruijff, B .

SCIENCE, 1999, 286 (5448) :2361-2364

[4] A NOVEL POSTTRANSLATIONAL MODIFICATION OF THE PEPTIDE ANTIBIOTIC SUBTILIN - ISOLATION AND CHARACTERIZATION OF A NATURAL VARIANT FROM BACILLUS-SUBTILIS ATCC-6633 [J].

CHAN, WC ;

BYCROFT, BW ;

LEYLAND, ML ;

LIAN, LY ;

ROBERTS, GCK .

BIOCHEMICAL JOURNAL, 1993, 291 :23-27

[5] Biosynthesis and mode of action of lantibiotics [J].

Chatterjee, C ;

Paul, M ;

Xie, LL ;

van der Donk, WA .

CHEMICAL REVIEWS, 2005, 105 (02) :633-683

[6] Enhanced nisin production by increasing genes involved in nisin Z biosynthesis in Lactococcus lactis subsp lactis A164 [J].

Cheigh, CI ;

Park, H ;

Choi, HJ ;

Pyun, YR .

BIOTECHNOLOGY LETTERS, 2005, 27 (03) :155-160

[7] Activation of subtilin precursors by Bacillus subtilis extracellular serine proteases subtilisin (AprE), WprA, and Vpr [J].

Corvey, C ;

Stein, T ;

Düsterhus, S ;

Karas, M ;

Entian, KD .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2003, 304 (01) :48-54

[8] REGULATION OF NISIN BIOSYNTHESIS AND IMMUNITY IN LACTOCOCCUS-LACTIS 6F3 [J].

ENGELKE, G ;

GUTOWSKIECKEL, Z ;

KIESAU, P ;

SIEGERS, K ;

HAMMELMANN, M ;

ENTIAN, KD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (03) :814-825

[9] DEVELOPMENTAL REGULATION OF TRANSCRIPTION OF THE BACILLUS-SUBTILIS FTSAZ OPERON [J].

GONZYTREBOUL, G ;

KARMAZYNCAMPELLI, C ;

STRAGIER, P .

JOURNAL OF MOLECULAR BIOLOGY, 1992, 224 (04) :967-979

[10] DEHYDROALANYLLYSINE - IDENTICAL COOH-TERMINAL STRUCTURES IN PEPTIDE ANTIBIOTICS NISIN AND SUBTILIN [J].

GROSS, E ;

MORELL, JL ;

CRAIG, LC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1969, 62 (03) :952-&

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