Preparation of insoluble transmembrane peptides: Glycophorin-A, prion (110-137), and FGFR (368-397)

被引：26

作者：

Glover, KJ ^{[1
]}

Martini, PM ^{[1
]}

Vold, RR ^{[1
]}

Komives, EA ^{[1
]}

机构：

[1] Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA

来源：

ANALYTICAL BIOCHEMISTRY | 1999年 / 272卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1006/abio.1999.4182

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A general procedure for the reliable preparation of large quantities of insoluble transmembrane peptides has been developed. Optimal couplings were obtained during synthesis by using high-temperature couplings in conjunction with O-(7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyluronium hexafluorophosphate (HATU) activation. Improved purification schemes were developed that use reverse- phase HPLC on a C1 column and elution with a 2:1 mixture of 1-propanol:1-butanol. Using these methods three very different transmembrane peptides all longer than 25 amino acids have been prepared: glycophorin-A, prion (110-137), and fibroblast growth factor receptor (368-397). (C) 1999 Academic Press.

引用

页码：270 / 274

页数：5

共 7 条

[1] Simple procedure for reversed-phase high-performance liquid chromatographic purification of long hydrophobic peptides that form transmembrane helices [J].

Lew, S ;

London, E .

ANALYTICAL BIOCHEMISTRY, 1997, 251 (01) :113-116

[2]

LLOYD DH, 1989, PEPTIDES CHEM STRUCT

[3] A transmembrane helix dimer: Structure and implications [J].

MacKenzie, KR ;

Prestegard, JH ;

Engelman, DM .

SCIENCE, 1997, 276 (5309) :131-133

[4]

RABINOVICH AK, 1994, PEPTIDES CHEM STRUCT

[5] STRUCTURE AND ORIENTATION OF THE TRANSMEMBRANE DOMAIN OF GLYCOPHORIN-A IN LIPID BILAYERS [J].

SMITH, SO ;

JONAS, R ;

BRAIMAN, M ;

BORMANN, BJ .

BIOCHEMISTRY, 1994, 33 (20) :6334-6341

[6] PREVENTION OF AGGREGATION OF SYNTHETIC MEMBRANE-SPANNING PEPTIDES BY ADDITION OF DETERGENT [J].

TOMICH, JM ;

CARSON, LW ;

KANES, KJ ;

VOGELAAR, NJ ;

EMERLING, MR ;

RICHARDS, JH .

ANALYTICAL BIOCHEMISTRY, 1988, 174 (01) :197-203

[7] Isotropic solutions of phospholipid bicelles: A new membrane mimetic for high-resolution NMR studies of polypeptides [J].

Vold, RR ;

Prosser, RS ;

Deese, AJ .

JOURNAL OF BIOMOLECULAR NMR, 1997, 9 (03) :329-335

← 1 →