Synaptic control of glycine and GABAA receptors and gephyrin expression in cultured motoneurons

被引:85
作者
Lévi, S
Chesnoy-Marchais, D
Sieghart, W
Triller, A
机构
[1] Ecole Normale Super, Lab Biol Cellulaire Synapse Normale & Pathol, INSERM, U497, F-75005 Paris, France
[2] Ecole Normale Super, Neurobiol Lab, CNRS, URA 1867, F-75005 Paris, France
[3] Univ Vienna, Psychiat Clin, Dept Biochem Psychiat, A-1090 Vienna, Austria
关键词
dorsal root ganglia; GABA(A) receptor; gephyrin; glycine receptor; motoneurons; picrotoxinin; presynaptic innervation; spinal neurons; strychnine;
D O I
10.1523/JNEUROSCI.19-17-07434.1999
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We have evaluated the influence of the secretory phenotype of presynaptic boutons on the accumulation of postsynaptic glycine receptors (GlyRs), type A GABA receptors (GABA(A)Rs), and gephyrin clusters. The cellular distribution of these components was analyzed on motoneurons cultured either alone or with glycinergic and/or GABAergic neurons. In motoneurons cultured alone, we observed gephyrin clusters at nonsynaptic sites and in front of cholinergic boutons, whereas glycine and GABA(A) receptors formed nonsynaptic clusters. These receptors are functionally and pharmacologically similar to those found in cultures of all spinal neurons. Motoneurons receiving GABAergic innervation from dorsal root ganglia neurons displayed postsynaptic clusters of gephyrin and GABA(A)R beta but not of GlyR alpha/beta subunits. In motoneurons receiving glycinergic and GABAergic innervation from spinal interneurons, gephyrin, GlyR alpha/beta, and GABA(A)R beta formed mosaics at synaptic loci. These results indicate that (1) the transmitter phenotype of the presynaptic element determines the postsynaptic accumulation of specific receptors but not of gephyrin and (2) the postsynaptic accumulation of gephyrin alone cannot account for the formation of GlyR-rich microdomains.
引用
收藏
页码:7434 / 7449
页数:16
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