The Rhizobium etli trpB gene is essential for an effective symbiotic interaction with Phaseolus vulgaris

被引：13

作者：

Taté, R ^{[1
]}

Riccio, A ^{[1
]}

Caputo, E ^{[1
]}

Cermola, M ^{[1
]}

Favre, R ^{[1
]}

Patriarca, EJ ^{[1
]}

机构：

[1] CNR, IIGB, I-80125 Naples, Italy

来源：

MOLECULAR PLANT-MICROBE INTERACTIONS | 1999年 / 12卷 / 10期

关键词：

trpFBA operon;

D O I：

10.1094/MPMI.1999.12.10.926

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A mutant strain (CTNUX4) of Rhizobium etli carrying Tn5 unable to grow with ammonium as the sole nitrogen source was isolated and characterized, Sequence analysis showed that Tn5 is inserted into a trpB (tryptophan synthase)-homologous gene. When tested on the roots of Phaseolus vulgaris, strain CTNUX4 was able to induce only small, slightly pink, ineffective (Fix(-)) nodules. However, under free-living conditions, strain CTNUX4 was unable to produce flavonoid-inducible lipo-chitin oligosaccharides (Nod factors) unless tryptophan was added to the growth medium. These data and histological observations indicate that the lack of tryptophan biosynthesis affects the symbiotic behavior of R. etli.

引用

页码：926 / 933

页数：8

共 26 条

[1]

[Anonymous], 1996, ESCHERICHIA COLI SAL

[2] RHIZOBIUM-MELILOTI MUTANTS UNABLE TO SYNTHESIZE ANTHRANILATE DISPLAY A NOVEL SYMBIOTIC PHENOTYPE [J].