Global Sequencing: A Review of Current Molecular Data and New Methods Available to Assess Microbial Diversity

被引：53

作者：

Christen, Richard ^{[1
,2
]}

机构：

[1] Univ Nice, F-06108 Nice, France

[2] Fac Sci, Lab Biol Virtuelle, Cente Biochim, CNRS, F-06108 Nice, France

来源：

MICROBES AND ENVIRONMENTS | 2008年 / 23卷 / 04期

关键词：

bacteria; diversity; 16S rRNA; pyrosequencing;

D O I：

10.1264/jsme2.ME08525

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Roughly estimating the diversity of life has for a long time been seen as a challenge. Sequences obtained from the environment by direct amplification are now seen as the sole mean to provide information for at least 99% of the prokaryotes in natural communities. Recent massive parallel sequencing technologies now allow to sequence nearly every sequence resulting from such amplification. Are we ready to deal with such a deluge of data? Using three recent publications (16S rRNA genes amplification in soil and seawater followed by massive parallel sequencing) as case examples, this review analyses the state of the field.

引用

页码：253 / 268

页数：16

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