Promoter protection by a transcription factor acting as a local topological homeostat

被引：36

作者：

Rochman, M

Aviv, M

Glaser, G

Muskhelishvili, G

机构：

[1] Max Planck Inst Terr Microbiol, D-35043 Marburg, Germany

[2] Hebrew Univ Jerusalem, Sch Med, Dept Cellular Biochem, IL-90101 Jerusalem, Israel

来源：

EMBO REPORTS | 2002年 / 3卷 / 04期

关键词：

D O I：

10.1093/embo-reports/kvf067

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Binding of the Escherichia coli global transcription factor FIS to the upstream activating sequence (UAS) of stable RNA promoters activates transcription on the outgrowth of cells from stationary phase. Paradoxically, while these promoters require negative supercoiling of DNA for optimal activity, FIS counteracts the increase of negative superhelical density by DNA gyrase. We demonstrate that binding of FIS at the UAS protects the rrnA P1 promoter from inactivation at suboptimal superhelical densities. This effect is correlated with FIS-dependent constraint of writhe and facilitated untwisting of promoter DNA. We infer that FIS maintains stable RNA transcription by stabilizing local writhe in the UAS. These results suggest a novel mechanism of transcriptional regulation by a transcription factor acting as a local topological homeostat.

引用

页码：355 / 360

页数：6

共 42 条

[1]

Aviv M, 1996, FEMS MICROBIOL LETT, V140, P71

[2] Regulation of rRNA transcription is remarkably robust:: FIS compensates for altered nucleoside triphosphate sensing by mutant RNA polymerases at Escherichia coli rrn P1 promoters [J].